Thursday June 17, 2010

Jeremy's gel electrophoresis of the colonies resulting from the construction of B0015 (double terminators) and R0040 (TetR repressible promoter)

Emily: Today I got sequencing results back for my I0500-B0034 construct. Unfortunately it does not look like the construction was succesful, as we couldn't find either part in the sequence. I restarted the construction, transformed it into TOP10 cells and plated on Kanymyacin for oveneright growth (with Dev's help) and tomorrow I shall start some more verification of these two parts seperately. Today I also looked into some more possible genes of interest for our various circuits. I also played around with creating a countdown to iGEM 2010 on our Wiki page.

Dev, Chris, Jeremy: Today, Jeremy restreaked the construct of B0015-R0040, did a PCR on it. He then ran a gel electrophoresis on the five colonies that grew. Colonies 1 and 5 are presumed to have the part desired as observed from the gel picture shown on the right. He also wrote a thank you letter to BioAlberta from which we received $1000 today. Dev helped Emily in her construction of I0500-B0034 through the Quick Ligase procedure. We are using presumed I0500 because we are unsure of the quality of the growth. I0500 has so far, failed to grow consistently. Chris redid the construction of J13002-E0032 to test the YFP from the Registry as well as attempting a plasmid switch of J23032 to a kanamycin resistant plasmid.