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- 14:07, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/buffers stock solutions (New page: ==Buffers and Stock solutions== '''Luria-bertani (LB)''' For 1 liter dissolve in H2O: {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" |Compoun...)
- 13:49, 5 July 2010 (diff | hist) Team:TU Delft/24 June 2010 content
- 13:43, 5 July 2010 (diff | hist) Team:TU Delft/protocols/midi-prep plasmid isolation
- 13:40, 5 July 2010 (diff | hist) Team:TU Delft/24 June 2010 content
- 13:39, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/colony PCR (New page: ==colony PCR== ''Materials:'' - Taq PCR Master Mix (Qiagen) is a premixed solution containing Taq DNA Polymerase, PCR Buffer, and dNTPs. The solution provides a final concentration of 1....)
- 13:20, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/agarose gel (New page: ==Agarose gel== 1. Dissolve 1 g of agarose in 100 mL 1x TBE buffer for 1% gel (can be stored in the 70 °C stove) 2. Add 5 µL of SYBRSafe (Invitrogen))
- 13:17, 5 July 2010 (diff | hist) Team:TU Delft/protocols/restriction enzyme digestion
- 13:17, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/ligation (New page: ==Ligation== Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier. React...)
- 13:16, 5 July 2010 (diff | hist) Team:TU Delft/protocols/restriction enzyme digestion
- 13:12, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/restriction enzyme digestion (New page: ==Restriction enzyme digestion== Digestions (cutting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to ...)
- 13:07, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/freezing bacterial stocks (New page: ==Freezing of bacterial stocks== ''Materials:'' - Bacterial culture - LB medium - 80% glycerol - Centrifuge ''Protocol:'' 1. Take 5 mL bacterial cells from the Erlenmeyer of a fre...)
- 13:05, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/midi-prep plasmid isolation (New page: ==Qiagen Midi-prep plasmid isolation== This protocol is based on QIAGEN® Plasmid Purification Handbook. This protocol is designed for preparation of up to 100 µg of high- or low-copy p...)
- 13:03, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/mini-prep plasmid isolation (New page: ==Qiagen Mini-prep plasmid isolation== This protocol is based on QIAGEN® Plasmid Purification Handbook. This protocol is designed for preparation of up to 20 µg of high-copy plasmid or ...)
- 12:56, 5 July 2010 (diff | hist) N File:Team-TU DelftTransformation efficiency1.TIF (top)
- 12:46, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/transformation (New page: ==Transformation== ''Materials:'' - competent cells - LB medium (warmed to room temperature) - DNA ligation mix - LB plates containing 15-100 μg/mL antibiotic of choice, pre-warmed t...)
- 12:38, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/provided biobricks (New page: == Use of Provided Biobricks== From the iGEM organization each team has received a distribution kit containing selected biobricks from the previous years. These biobricks are provided in ...)
- 12:33, 5 July 2010 (diff | hist) Team:TU Delft/protocols/making competent cells
- 12:29, 5 July 2010 (diff | hist) Team:TU Delft/protocols/making competent cells
- 12:29, 5 July 2010 (diff | hist) N Team:TU Delft/protocols/making competent cells (New page: === Making competent cells=== ''Materials:'' - bacterial culture - centrifuge - 0.1 M MgCl2 - 0.1 M CaCl2 - 80% glycerol ''Protocol:'' 1. Cultivate 100 mL LB medium, 37 °C o/n ...)
- 12:16, 5 July 2010 (diff | hist) Team:TU Delft/18 June 2010 content
- 12:15, 5 July 2010 (diff | hist) Team:TU Delft/18 June 2010 content
- 12:09, 5 July 2010 (diff | hist) Team:TU Delft/12 June 2010 content
- 11:56, 5 July 2010 (diff | hist) Team:TU Delft/6 June 2010 content
- 11:54, 5 July 2010 (diff | hist) Team:TU Delft/3 June 2010 content
- 11:53, 5 July 2010 (diff | hist) Team:TU Delft/3 June 2010 content
- 11:53, 5 July 2010 (diff | hist) Team:TU Delft/3 June 2010 content
- 11:51, 5 July 2010 (diff | hist) Team:TU Delft/22 June 2010 content
- 11:49, 5 July 2010 (diff | hist) Team:TU Delft/23 June 2010 content
- 11:46, 5 July 2010 (diff | hist) Team:TU Delft/23 June 2010 content
- 11:43, 5 July 2010 (diff | hist) Team:TU Delft/5 July 2010 (top)
- 11:43, 5 July 2010 (diff | hist) Team:TU Delft/5 July 2010 (Removing all content from page)
- 11:42, 5 July 2010 (diff | hist) Team:TU Delft/5 July 2010 content
- 11:41, 5 July 2010 (diff | hist) N Team:TU Delft/5 July 2010 (New page: The exams are finally over: In-vivo biomolecular interactions underlying diseases, aged based modeling of complex adaptive systems, biophysics of protein and protein interactions, function...)
- 15:35, 30 June 2010 (diff | hist) N Team:TU Delft/30 June 2010 (New page: {{:Team:TU_Delft/calendar_page|day=30|month=June}}) (top)
- 15:34, 30 June 2010 (diff | hist) N Team:TU Delft/29 June 2010 (New page: {{:Team:TU_Delft/calendar_page|day=29|month=June}}) (top)
- 15:21, 30 June 2010 (diff | hist) N Team:TU Delft/30 June 2010 content (New page: We harvested the 50 mL bacterial cells of the 15 biobricks from the distribution plates. We used 3 mL of the baceterial cells to make -80 °C stocks. With the rest we performed a Qiagen Mi...)
- 15:19, 30 June 2010 (diff | hist) N Team:TU Delft/29 June 2010 content (New page: We are a bit short handed in the lab this week, because most team members have exams. Considering the fine weather outside, it is not bad to have a slow week. We still managed to pick col...)
- 14:05, 24 June 2010 (diff | hist) Team:TU Delft/24 June 2010 content
- 13:45, 24 June 2010 (diff | hist) Team:TU Delft/24 June 2010 (top)
- 13:44, 24 June 2010 (diff | hist) N Team:TU Delft/24 June 2010 (New page: __NOTOC__ {{:Team:TU_Delft/header}} <html> <div id="menu_wrap" class="blog"> </html> {{:Team:TU_Delft/menu}} <html> </div> <div id="content_wrap"> <table id="content_table"> <tr> <td id="t...)
- 13:42, 24 June 2010 (diff | hist) Team:TU Delft/21 June 2010 content (→Lab work)
- 13:37, 24 June 2010 (diff | hist) Team:TU Delft/21 June 2010 content
- 13:37, 24 June 2010 (diff | hist) Team:TU Delft/18 June 2010 content
- 13:30, 24 June 2010 (diff | hist) Team:TU Delft/21 June 2010 content
- 13:05, 24 June 2010 (diff | hist) Team:TU Delft/21 June 2010 content
- 13:50, 22 June 2010 (diff | hist) N Team:TU Delft/21 June 2010 (New page: __NOTOC__ {{:Team:TU_Delft/header}} <html> <div id="menu_wrap" class="blog"> </html> {{:Team:TU_Delft/menu}} <html> </div> <div id="content_wrap"> <table id="content_table"> <tr> <td id="t...)
- 13:49, 22 June 2010 (diff | hist) N Team:TU Delft/21 June 2010 content (New page: We had some drawbacks today. The gel extraction kit didn’t work as well as we had hoped. Tomorrow a new day.)
- 13:49, 22 June 2010 (diff | hist) Team:TU Delft/22 June 2010
- 13:47, 22 June 2010 (diff | hist) N Team:TU Delft/22 June 2010 content (New page: We tried to compensate for yesterdays delays. We transformed 15 Biobricks from the distribution plates!)
- 13:46, 22 June 2010 (diff | hist) N Team:TU Delft/22 June 2010 (New page: We tried to compensate for yesterdays delays. We transformed 15 Biobricks from the distribution plates!)
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