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- 19:48, 29 July 2010 (diff | hist) N Week of 6/14 (New page: <center>Image:6-14-10.jpg</center>)
- 19:44, 29 July 2010 (diff | hist) N File:7-12-10.jpg (top)
- 19:43, 29 July 2010 (diff | hist) N File:7-9-10.jpg (top)
- 19:42, 29 July 2010 (diff | hist) N File:7-8-10.jpg (top)
- 19:41, 29 July 2010 (diff | hist) N File:7-7-10.jpg (top)
- 19:39, 29 July 2010 (diff | hist) N File:7-2-10.jpg (top)
- 19:38, 29 July 2010 (diff | hist) N File:7-1-10.jpg (top)
- 19:36, 29 July 2010 (diff | hist) N File:6-30-10 001.jpg (top)
- 19:31, 29 July 2010 (diff | hist) N File:6-30-10.jpg (top)
- 19:30, 29 July 2010 (diff | hist) N File:6-29-10.jpg (top)
- 19:29, 29 July 2010 (diff | hist) N File:6-25-10.jpg (top)
- 19:28, 29 July 2010 (diff | hist) N File:6-24-10.jpg (top)
- 19:26, 29 July 2010 (diff | hist) N File:6-22-10 001.jpg (top)
- 19:25, 29 July 2010 (diff | hist) N File:6-22-10.jpg (top)
- 19:23, 29 July 2010 (diff | hist) N File:6-17-10.jpg (top)
- 19:21, 29 July 2010 (diff | hist) N File:6-16-10.jpg (top)
- 19:19, 29 July 2010 (diff | hist) N File:6-15-10.jpg (top)
- 19:17, 29 July 2010 (diff | hist) N File:6-14-10.jpg (top)
- 19:13, 29 July 2010 (diff | hist) Week of 6/7
- 19:11, 29 July 2010 (diff | hist) Week of 6/7
- 19:11, 29 July 2010 (diff | hist) N File:6-10-10 001.jpg (top)
- 19:09, 29 July 2010 (diff | hist) Week of 6/7
- 19:09, 29 July 2010 (diff | hist) N File:6-10-10.jpg (top)
- 19:03, 29 July 2010 (diff | hist) Week of 6/7
- 19:00, 29 July 2010 (diff | hist) N File:6-9-10.jpg (top)
- 18:52, 29 July 2010 (diff | hist) N Week of 6/7 (New page: *Flasks mini prepped -K091107 (pLux/cI Hybrid Promoter) -R0061 (Promoter (HSL-mediated luxR repressor) -R0063 (Promoter (luxR & HSL regulated -- lux pL) -K091146 (pLas/...)
- 18:37, 29 July 2010 (diff | hist) N File:6-4-10.jpg (top)
- 17:52, 29 July 2010 (diff | hist) N Week of 5/31 (New page: Week of 5/31 *Flasks Mini prepped: - B0010 (Transcriptional Terminator) - R0062 (Promoter activated by LuxR in concert with HSL) - C0012 (lacI repressor from E. coli) - R0...)
- 15:37, 20 July 2010 (diff | hist) N Preparing Electrocompetent E. Coli Cells (New page: Day 1 1.Inoculate 25 mL of LB medium with the cell line you want and grow overnight with rotation at 37 degrees Celsius. 2.Autoclave the following: a.Eight 125 mL flasks containing ...) (top)
- 15:10, 20 July 2010 (diff | hist) Protocols
- 15:09, 20 July 2010 (diff | hist) N Electrotransformation of E. Coli (New page: 1.Chill electroporation cuvettes on ice 2.Prepare the SOC media by adding MgCl2 and Glucose solutions to prepare SOB media and pipette 900 ul into the desired amount of recovery vials 3....) (top)
- 14:59, 20 July 2010 (diff | hist) Protocols
- 14:58, 20 July 2010 (diff | hist) N Ligation (New page: 1. Mix together the following: a. 5 ul deionized water b. 2 ul of Reaction Buffer c. 5 ul of digested downstream insert (or water if using only 1 insert) d. 5 ul of dig...)
- 14:56, 20 July 2010 (diff | hist) Protocols
- 14:55, 20 July 2010 (diff | hist) N DNA Digest (New page: 50 ul digest 1. Quickly vortex all ingredients (Buffer, BSA, DNA, Enzymes) before beginning. 2. Add the following in a micro-centrifuge tube: a.5 ul of Buffer 2 b.1 u...) (top)
- 14:49, 20 July 2010 (diff | hist) Protocols
- 14:36, 20 July 2010 (diff | hist) Plasmid Mini Prep (top)
- 14:35, 20 July 2010 (diff | hist) N Plasmid Mini Prep (New page: Mini Prep 1.Isolate single colony from a streaked plate and inoculate a culture. a.Incubate for ~ 12-16 hrs 2.Pellet bacterial cells by centri...)
- 14:16, 20 July 2010 (diff | hist) Protocols
- 19:21, 19 July 2010 (diff | hist) Making Media
- 19:15, 19 July 2010 (diff | hist) N Making Media (New page: LB media * 10 g LB broth * 500 mL DI water autoclave to sterilize LB media with agar * 10 g LB broth * 7.5 g agar * 500 mL DI water autoclave to sterilize add a...)
- 19:13, 19 July 2010 (diff | hist) Team:Penn State/Notebook (→Notebook)
- 19:06, 19 July 2010 (diff | hist) Team:Penn State/Notebook (→Notebook)
- 19:03, 19 July 2010 (diff | hist) N Colony PCR (New page: Colony PCR 1. Taq 10X buffer (each tube) a.36 ul H2O b.5 ul buffer thermopole c.1 ul DNTp d.1 ul primer 1 (Brk primer) e.1 ul p...) (top)
- 19:01, 19 July 2010 (diff | hist) N Protocols (New page: Colony PCR)
- 18:59, 19 July 2010 (diff | hist) Team:Penn State/Notebook (→Notebook)
- 18:58, 19 July 2010 (diff | hist) N Daily Schedule- Notebook (New page: '''Daily Schedule''' 1. Miniprep Flasks (1 hr) 2. Restriction Digest (1 hr) A)Standard Assembly i.Small part EX or SP digest ii.Large Part XP or ES digest a...)
- 18:05, 19 July 2010 (diff | hist) Team:Penn State/Notebook (→Notebook)
- 17:51, 19 July 2010 (diff | hist) Team:Penn State/Notebook (→Notebook)
- 15:47, 19 July 2010 (diff | hist) Team:Penn State/Notebook (→Notebook)
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