Team:HokkaidoU Japan/Notebook/August27

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高橋欠席のため記録無し．
+
ラオリ英語添削お願い
 +
 
 +
=Transfer 1-1A on pSB1A10 to pSB1C3=
 +
1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before
 +
2.mixed solutions according to the table below
 +
 
 +
{|style="text-align: center;" class="protocol"
 +
|-
 +
!Reagent
 +
!Amount
 +
|-
 +
|pSB1C3
 +
|5 uL
 +
|-
 +
|DW
 +
|75 uL
 +
|-
 +
|10x M Buffer
 +
|10 uL
 +
|-
 +
|1%BSA
 +
|10 uL
 +
|-
 +
|EcoRI
 +
|5 uL
 +
|-
 +
|PstI
 +
|5 uL
 +
|-
 +
|style="border-top:1px solid #996"|'''Total'''
 +
|style="border-top:1px solid #996"|'''100 uL'''
 +
|}
 +
{|style="text-align: center;" class="protocol"
 +
|-
 +
!Reagent
 +
!Amount
 +
|-
 +
|1-1A
 +
|8 uL
 +
|-
 +
|DW
 +
|6 uL
 +
|-
 +
|10x M Buffer
 +
|2 uL
 +
|-
 +
|1%BSA
 +
|2 uL
 +
|-
 +
|EcoRI
 +
|1 uL
 +
|-
 +
|PstI
 +
|1 uL
 +
|-
 +
|style="border-top:1px solid #996"|'''Total'''
 +
|style="border-top:1px solid #996"|'''20 uL'''
 +
|}

Revision as of 11:58, 13 October 2010

Notebook

ラオリ英語添削お願い

Transfer 1-1A on pSB1A10 to pSB1C3

1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before 2.mixed solutions according to the table below

Reagent Amount
pSB1C3 5 uL
DW 75 uL
10x M Buffer 10 uL
1%BSA 10 uL
EcoRI 5 uL
PstI 5 uL
Total 100 uL
Reagent Amount
1-1A 8 uL
DW 6 uL
10x M Buffer 2 uL
1%BSA 2 uL
EcoRI 1 uL
PstI 1 uL
Total 20 uL
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Notebook/August27"