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Talk:Team:IvyTech-South Bend/21 September 2010
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Rchamberlin (Talk | contribs)
(New page: == 9/21/10 == Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23 1) I will put frozen electro comp cells from -80 degrees C freezer 2) We wil...)
Newer edit →
(New page: == 9/21/10 == Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23 1) I will put frozen electro comp cells from -80 degrees C freezer 2) We wil...)
Newer edit →
Revision as of 13:49, 12 October 2010
9/21/10
Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23
1) I will put frozen electro comp cells from -80 degrees C freezer
2) We will do two “2” electroporations with (AT) one with 5 mL T9002 Gene, and one with 20 mL
3) Icing Cuvette (.1) and now prepared to electroporate
4) Reading for 20 mL was 2.2 kv @ 0.70 ms
5) then removed with 1 mL SOB media
6) Placed into 1.5 mL Sterile Centrifuge tube at room temp for 3 hrs prof T. will streak.
