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Team:Harvard/flavor/parts
From 2010.igem.org
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| + | <h1>parts and primers</h1> | ||
| + | <h2>Primers</h2> | ||
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| + | <tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr> | ||
| + | <tr><td>J45004_F</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr> | ||
| + | <tr><td>J45004_R</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td></td></tr> | ||
| + | <tr><td>J45017_F</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr> | ||
| + | <tr><td>J45017_R</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | ||
| + | <tr><td>NOSterm_BB_F</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr> | ||
| + | <tr><td>NOSterm_BB_R</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | ||
| + | <tr><td>Stop_NOSterm_BB_F</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr> | ||
| + | <tr><td>pENTCUP2_BB_F</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr> | ||
| + | <tr><td>pENTCUP2_BB_R</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr> | ||
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Revision as of 15:33, 10 October 2010
parts and primers
Primers
| Name | Description | Sequence |
| J45004_F | HindIII-pENTCUP2 forward primer for Expression Series insert | |
| J45004_R | pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert | |
| J45017_F | HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert | |
| J45017_R | gusA-NheI reverse primer for Reporter Series 1 insert | |
| NOSterm_BB_F | HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert | |
| NOSterm_BB_R | smGFP-NheI reverse primer for Reporter Series 1 insert | |
| Stop_NOSterm_BB_F | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| pENTCUP2_BB_F | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| pENTCUP2_BB_R | 1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter |
