Team:Edinburgh/Notebook/Blue light producer

From 2010.igem.org

(Difference between revisions)
(→Blue Light Producer)
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| LuxAB  
| LuxAB  
|}
|}
 +
 +
'''GEL PIC'''
 +
{| border="1"
 +
| Lane 1
 +
| Lane 2 +3
 +
| Lanes 3-6
 +
|-
 +
| Ladder
 +
| EdiI
 +
| LuxAB
 +
|}
 +
 +
* The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working
 +
 +
'''29/7/2010'''

Revision as of 12:19, 7 October 2010





Blue Light Producer


13/7/2010

Empty edinbrick vector for control.

  • LuxAB + edinbrick ligation.

28/7/2010

an update on LuxAB:

  • The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
  • Still have some digested LuxAB-ediI so re-did ligation and transformation.

Gel Pic- minipreps

Lane 1&15 Lane 2 Others
Ladder EdiI LuxAB

GEL PIC

Lane 1 Lane 2 +3 Lanes 3-6
Ladder EdiI LuxAB
  • The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working

29/7/2010

Retrieved from "http://2010.igem.org/Team:Edinburgh/Notebook/Blue_light_producer"