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O/N Culture
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(New page: '''REMEMBER TO BE STERILE''' *Take 5mL LB and put in a round bottom tube *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube '''MAKE SURE YOU ADD THE RIGHT ...)
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(New page: '''REMEMBER TO BE STERILE''' *Take 5mL LB and put in a round bottom tube *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube '''MAKE SURE YOU ADD THE RIGHT ...)
Newer edit →
Revision as of 17:36, 14 June 2010
REMEMBER TO BE STERILE
- Take 5mL LB and put in a round bottom tube
- If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube MAKE SURE YOU ADD THE RIGHT ONE!
- Take a tip on a p200 and lightly touch a colony
- Eject the tip into the culture and put the cap on
- For a miniprep, grow O/N for 12-16 hours NOT LONGER
