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Team:DTU-Denmark/Wet lab
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!align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Gallery|<span style="color:white;">Gallery</span>]] | !align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Gallery|<span style="color:white;">Gallery</span>]] | ||
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| + | = Wetlab = | ||
| + | On this page is described the experiences, procedures and protocols that we have used. | ||
| + | Further the experimental process is described for the two parallel tracks, the repressor and terminator part, what have we experienced when working with our parts, and the BB standard. | ||
| + | |||
| + | == Repressor group == | ||
| + | |||
| + | == Terminator group == | ||
| + | [ here we should write a short abstract ] | ||
| + | |||
| + | === Work flow === | ||
| + | |||
| + | |||
| + | === References === | ||
| + | * | ||
| + | |||
| + | |||
= Anti-Terminator experimental setup = | = Anti-Terminator experimental setup = | ||
Revision as of 20:13, 2 October 2010
 | Home | Team | Project | Parts Submitted to the Registry | Modeling | Wet Lab | Gallery |
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Contents |
Wetlab
On this page is described the experiences, procedures and protocols that we have used. Further the experimental process is described for the two parallel tracks, the repressor and terminator part, what have we experienced when working with our parts, and the BB standard.
Repressor group
Terminator group
[ here we should write a short abstract ]
Work flow
References
Anti-Terminator experimental setup
N-protein plasmid
The N protein were isolated from salmonella genomic DNA with specific designed primers. We used the natural occurring RBS site, as a High expression of N have shown non specific anti-termination effect on a global scale on the genome. #References References
References
- NC Franklin, JH Doelling - Am Soc Microbiol "Overexpression of N antitermination proteins of bacteriophages lambda, 21, and P22: loss of N protein specificity." - Journal of bacteriology, 1989
