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Team:Edinburgh/Project
From 2010.igem.org
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<p>Our BRIDGE construct will contain chloramphenicol resistance (<i>cat</i>) and <i>sacB</i>. Both it and the desired gene will be inserted by homologous recombination using the lambda red system. For this we will need up and down-stream sequences of genes which we wish to replace.</p> | <p>Our BRIDGE construct will contain chloramphenicol resistance (<i>cat</i>) and <i>sacB</i>. Both it and the desired gene will be inserted by homologous recombination using the lambda red system. For this we will need up and down-stream sequences of genes which we wish to replace.</p> | ||
<p>To prove the principle of BRIDGE we will remove a non-essential, constitutively expressed gene from the <i>E. coli</i> genome and replace it with a well known marker, such as GFP. We also have several genes from a past project idea which we could delete to increase fatty acid synthesis, and further genes we could introduce which will result in the production of long chain alkenes from the excess fatty acids. This is not useful for our current project but it is a nice way to demonstrate the effectiveness of BRIDGE.</p> | <p>To prove the principle of BRIDGE we will remove a non-essential, constitutively expressed gene from the <i>E. coli</i> genome and replace it with a well known marker, such as GFP. We also have several genes from a past project idea which we could delete to increase fatty acid synthesis, and further genes we could introduce which will result in the production of long chain alkenes from the excess fatty acids. This is not useful for our current project but it is a nice way to demonstrate the effectiveness of BRIDGE.</p> | ||
| - | <p>Eventually, we hope that BRIDGE will be used to introduce whole light producer-sensor constructs, to demonstrate its ability for utilisation in further work using BioBricks.</p> | + | <p>Eventually, we hope that BRIDGE will be used to introduce whole light producer-sensor constructs, to demonstrate its ability for utilisation in further work using BioBricks.</p><br> |
| + | <br> | ||
<a name="Content" id="Content"></a><h2>Table of Contents</h2> | <a name="Content" id="Content"></a><h2>Table of Contents</h2> | ||
Revision as of 15:02, 30 September 2010
Genomic BRIDGEs
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Our Project
Our BRIDGE construct will contain chloramphenicol resistance (cat) and sacB. Both it and the desired gene will be inserted by homologous recombination using the lambda red system. For this we will need up and down-stream sequences of genes which we wish to replace.
To prove the principle of BRIDGE we will remove a non-essential, constitutively expressed gene from the E. coli genome and replace it with a well known marker, such as GFP. We also have several genes from a past project idea which we could delete to increase fatty acid synthesis, and further genes we could introduce which will result in the production of long chain alkenes from the excess fatty acids. This is not useful for our current project but it is a nice way to demonstrate the effectiveness of BRIDGE.
Eventually, we hope that BRIDGE will be used to introduce whole light producer-sensor constructs, to demonstrate its ability for utilisation in further work using BioBricks.
Table of Contents
- The protocol proper, explaining the technical details of BRIDGE.
- The BioBricks we submitted as part of developing the BRIDGE protocol.
- A summary of what we achieved as part of developing the BRIDGE protocol.
- Our vision of the future of the BRIDGE protocol, and where we would like to go next.
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References used throughout the section.
