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Team:UNIPV-Pavia/Calendar/September/settimana5
From 2010.igem.org
(Difference between revisions)
m (→September, 27th) |
(→September, 29th) |
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Miniprep and quantification for: | Miniprep and quantification for: | ||
| - | *I1: | + | *I1: 59,6 ng/ul |
| - | *I4: | + | *I4: 109,9 ng/ul |
| - | *I53: | + | *I53: 160,3 ng/ul |
| - | *<partinfo>BBa_J04450</partinfo>-1: | + | *<partinfo>BBa_J04450</partinfo>-1:55,9 ng/ul |
| - | *<partinfo>BBa_J04450</partinfo>-2: | + | *<partinfo>BBa_J04450</partinfo>-2: 47 ng/ul |
ON digestion of: | ON digestion of: | ||
| - | *I1 () | + | *I1 (E-S) |
| - | *I4 () | + | *I4 (E-S) |
*I53 (E-X) | *I53 (E-X) | ||
| - | *<partinfo>BBa_J04450</partinfo>-1 () | + | *<partinfo>BBa_J04450</partinfo>-1 (E-P) |
| - | *<partinfo>BBa_J04450</partinfo>-2 () | + | *<partinfo>BBa_J04450</partinfo>-2 (E-P) |
| - | *MyYeast-3 (DNA already available) () | + | *MyYeast-3 (DNA already available) (E-S) |
| - | *<partinfo>pSB4C5</partinfo> (DNA already available) () | + | *<partinfo>pSB4C5</partinfo> (DNA already available) (E-P) |
| + | |||
| + | Gel run for all digested parts and gel run/cut/purification for: | ||
| + | *4C5 (E-P): 19,3 ng/ul | ||
| + | *My Yeast 3 (E-S): 20,8 ng/ul | ||
| + | *I4 (E-S): 14,2 ng/ul | ||
| + | *I53 (E-X): 21,6 ng/ul | ||
| + | <partinfo>BBa_J04450</partinfo>(pLac-RBS-mRFP-TT) (E-P): 5,6 ng/ul | ||
| + | *<partinfo>pSB1C3</partinfo> 10 ng/ul | ||
| + | *I1: 3,8 ng/ul | ||
| + | |||
| + | |||
| + | Ligation of: | ||
| + | {| align='center', border='1' | ||
| + | | I70=MyYeast 3(E-S)+I4(E-S) | ||
| + | |- | ||
| + | | I71=MyYeast 3(E-S)+I1(E-S) | ||
| + | |- | ||
| + | |I72=<partinfo>BBa_J04450</partinfo>(E-P)+<partinfo>pSB4C5</partinfo> | ||
| + | |- | ||
| + | I73= | ||
| + | |} | ||
| + | |||
Revision as of 09:16, 30 September 2010
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