Team:UNIPV-Pavia/Freezer

From 2010.igem.org

(Difference between revisions)
m
Line 1: Line 1:
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 +
<table width="100%" border="0">
<table width="100%" border="0">
<tr>
<tr>
Line 306: Line 308:
<!--Ligazioni fatte durante l'iGEM2010 -->
<!--Ligazioni fatte durante l'iGEM2010 -->
<tr>
<tr>
-
<th>Ligation name</th>
+
<th>Name</th>
-
<th>Description</th>
+
<th>Ligation</th>
<th>Creation date</th>
<th>Creation date</th>
<th>Strain</th>
<th>Strain</th>
Line 313: Line 315:
<th>Quality control</th>
<th>Quality control</th>
<th>Length</th>
<th>Length</th>
-
<th>Store</th>
+
<th>Description</th>
</tr>
</tr>
Line 332: Line 334:
Seq ok (BMR); length ok.
Seq ok (BMR); length ok.
</td>  
</td>  
-
<td>854 bp</td><td>Ligations 2010</td>
+
<td>854 bp</td>
 +
<td>GFP silver (GFPs)</td>
</tr>
</tr>
Line 352: Line 355:
</td>  
</td>  
<td>1029 bp</td>
<td>1029 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 373: Line 376:
Seq ok (BMR); Cm resistance ok; length ok.
Seq ok (BMR); Cm resistance ok; length ok.
</td>  
</td>  
-
<td>906 bp</td><td>Ligations 2010</td>
+
<td>906 bp</td><td></td>
</tr>
</tr>
Line 394: Line 397:
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok.
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok.
</td>  
</td>  
-
<td>801 bp</td><td>Ligations 2010</td>
+
<td>801 bp</td><td></td>
</tr>
</tr>
Line 415: Line 418:
</td>  
</td>  
<td>1439 bp</td>
<td>1439 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 434: Line 437:
Seq ok (BMR); Cm resistance ok; length ok.
Seq ok (BMR); Cm resistance ok; length ok.
</td>  
</td>  
-
<td>1320 bp</td><td>Ligations 2010</td>
+
<td>1320 bp</td><td></td>
</tr>
</tr>
Line 454: Line 457:
</td>  
</td>  
<td>2754 bp</td>
<td>2754 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 476: Line 479:
</td>  
</td>  
<td>2795 bp</td>
<td>2795 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 498: Line 501:
</td>  
</td>  
<td>2795 bp</td>
<td>2795 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 519: Line 522:
</td>  
</td>  
<td>2795 bp</td>
<td>2795 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 541: Line 544:
</td>  
</td>  
<td>2795 bp</td>
<td>2795 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 565: Line 568:
</td>  
</td>  
<td>2795 bp</td>
<td>2795 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 589: Line 592:
</td>  
</td>  
<td>842 bp</td>
<td>842 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 615: Line 618:
1328 bp
1328 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 638: Line 641:
</td>  
</td>  
<td>842 bp</td>
<td>842 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 662: Line 665:
</td>  
</td>  
<td>842 bp</td>
<td>842 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 685: Line 688:
</td>  
</td>  
<td>842 bp</td>
<td>842 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 709: Line 712:
</td>  
</td>  
<td>842 bp</td>
<td>842 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 733: Line 736:
</td>  
</td>  
<td>842 bp</td>
<td>842 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 744: Line 747:
</td>
</td>
<td>
<td>
-
Pha-10S-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana5#July.2C_29th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S)
+
Pha10S-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana5#July.2C_29th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S)
</td>
</td>
<td>
<td>
Line 761: Line 764:
576 bp
576 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>Pha10S</td>
</tr>
</tr>
Line 772: Line 775:
</td>
</td>
<td>
<td>
-
Pha-SS-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana2#August.2C_10th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1A3</partinfo> (X-S)
+
PhaSS-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana2#August.2C_10th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1A3</partinfo> (X-S)
</td>
</td>
<td>
<td>
Line 786: Line 789:
576 bp
576 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>PhaSS</td>
</tr>
</tr>
Line 811: Line 814:
825 bp
825 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 836: Line 839:
725 bp
725 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 863: Line 866:
886 bp
886 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 890: Line 893:
612 bp
612 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>linker+PhaS</td>
</tr>
</tr>
Line 918: Line 921:
2678 bp
2678 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 946: Line 949:
2839 bp
2839 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 974: Line 977:
612 bp
612 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>Pha10S+linker</td>
</tr>
</tr>
Line 1,001: Line 1,004:
1158 bp
1158 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>Pha10S+PhaSS</td>
</tr>
</tr>
Line 1,027: Line 1,030:
1436 bp
1436 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>Pha10S+GFPs</td>
</tr>
</tr>
Line 1,054: Line 1,057:
1436 bp
1436 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>PhaSS+GFPs</td>
</tr>
</tr>
Line 1,081: Line 1,084:
612 bp
612 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>PhaSS+linker</td>
</tr>
</tr>
Line 1,108: Line 1,111:
1158 bp
1158 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>PhaSS+PhaSS</td>
</tr>
</tr>
Line 1,135: Line 1,138:
1367 bp
1367 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>INTEIN+GFPs</td>
</tr>
</tr>
Line 1,162: Line 1,165:
1079 bp
1079 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>HSL inducible system</td>
</tr>
</tr>
Line 1,188: Line 1,191:
652 bp
652 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>pTet+Pha10S</td>
</tr>
</tr>
Line 1,215: Line 1,218:
1125 bp
1125 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>linker+PhaSS+INTEIN</td>
</tr>
</tr>
Line 1,242: Line 1,245:
1472 bp
1472 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 1,268: Line 1,271:
3068 bp
3068 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 1,294: Line 1,297:
3026 bp
3026 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 1,321: Line 1,324:
3239 bp
3239 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 1,348: Line 1,351:
2149 bp
2149 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 1,376: Line 1,379:
1517 bp
1517 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>pTet+Pha10S+GFPs</td>
</tr>
</tr>
Line 1,404: Line 1,407:
2094 bp
2094 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>pTet+Pha10S+PhaSS+GFPs</td>
</tr>
</tr>
Line 1,431: Line 1,434:
2130 bp
2130 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>pTet+Pha10S+linker+PhaSS+GFPs</td>
</tr>
</tr>
Line 1,458: Line 1,461:
3239 bp
3239 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 1,487: Line 1,490:
3239 bp
3239 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 1,515: Line 1,518:
1985 bp
1985 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>PhaSS+linker+INTEIN+GFPs</td>
</tr>
</tr>
Line 1,543: Line 1,546:
2531 bp
2531 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>Pha10S+PhaSS+INTEIN+GFPs</td>
</tr>
</tr>
Line 1,571: Line 1,574:
2531 bp
2531 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>PhaSS+PhaSS+INTEIN+GFPs</td>
</tr>
</tr>
Line 1,599: Line 1,602:
1661 bp
1661 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td><partinfo>BBa_F2620</partinfo>+Pha10S</td>
</tr>
</tr>
Line 1,628: Line 1,631:
2090 bp
2090 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>linker+PhaSS+linker+PhaSS+GFPs</td>
</tr>
</tr>
Line 1,657: Line 1,660:
1985 bp
1985 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>linker+PhaSS+GFPs</td>
</tr>
</tr>
Line 1,686: Line 1,689:
1697 bp
1697 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td><partinfo>BBa_F2620</partinfo>+Pha10S+linker</td>
</tr>
</tr>
Line 1,713: Line 1,716:
2018 bp
2018 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>PhaSS+PhaSS+GFPs</td>
</tr>
</tr>
Line 1,740: Line 1,743:
1194 bp
1194 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>Pha10S+linker+PhaSS</td>
</tr>
</tr>
Line 1,767: Line 1,770:
1985 bp
1985 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>Pha10S+linker+INTEIN+GFPs</td>
</tr>
</tr>
Line 1,773: Line 1,776:
<tr>
<tr>
<td>
<td>
-
I62
+
<font class="BBok">I62</font>
<br/>
<br/>
-
(it was I62-)
+
(it was I62-E)
<br/>
<br/>
</td>
</td>
Line 1,782: Line 1,785:
</td>
</td>
<td>
<td>
-
 
+
27/09/2010
</td><td>
</td><td>
''E. coli'' TOP10
''E. coli'' TOP10
Line 1,790: Line 1,793:
</td>
</td>
<td>
<td>
-
 
+
Length ok.
</td>  
</td>  
<td>
<td>
2678 bp
2678 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>pTet+Pha10S+PhaSS+PhaSS+GFPs</td>
</tr>
</tr>
Line 1,823: Line 1,826:
3070 bp
3070 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td><partinfo>BBA_F2620</partinfo>+Pha10S+linker+INTEIN+GFPs</td>
</tr>
</tr>
Line 1,851: Line 1,854:
3688 bp
3688 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td><partinfo>BBA_F2620</partinfo>+Pha10S+linker+PhaSS+linker+INTEIN+GFPs</td>
</tr>
</tr>
Line 1,879: Line 1,882:
2748 bp
2748 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>pTet+Pha10S+linker+PhaSS+linker+PhaSS+GFPs</td>
</tr>
</tr>
Line 1,907: Line 1,910:
3185 bp
3185 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td>Pha10S+linker+PhaSS+linker+PhaSS+INTEIN+GFPs</td>
</tr>
</tr>
Line 1,932: Line 1,935:
507 bp
507 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 1,956: Line 1,959:
1695 bp
1695 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 1,981: Line 1,984:
829 bp
829 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,009: Line 2,012:
2149 bp
2149 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,032: Line 2,035:
</td>  
</td>  
<td>2795 bp</td>
<td>2795 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,055: Line 2,058:
</td>  
</td>  
<td>2795 bp</td>
<td>2795 bp</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,079: Line 2,082:
2795 bp
2795 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,104: Line 2,107:
2795 bp
2795 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,129: Line 2,132:
842 bp
842 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,153: Line 2,156:
842 bp
842 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,178: Line 2,181:
842 bp
842 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,205: Line 2,208:
842 bp
842 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,230: Line 2,233:
842 bp
842 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,255: Line 2,258:
842 bp
842 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,281: Line 2,284:
725 bp
725 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,306: Line 2,309:
886 bp
886 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,331: Line 2,334:
904 bp
904 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,355: Line 2,358:
904 bp
904 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,379: Line 2,382:
904 bp
904 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,404: Line 2,407:
904 bp
904 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,428: Line 2,431:
904 bp
904 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,452: Line 2,455:
904 bp
904 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,476: Line 2,479:
904 bp
904 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>
Line 2,502: Line 2,505:
904 bp
904 bp
</td>
</td>
-
<td>Ligations 2010</td>
+
<td></td>
</tr>
</tr>

Revision as of 20:59, 29 September 2010
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FREEZER MANAGEMENT


Biobrick name Description Creation date Strain Vector Quality control Length Store

<partinfo>BBa_E2050</partinfo>

mOrange

08/06/2010

E. coli DH5-alpha

<partinfo>pSB2K3</partinfo>

Seq ok (MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>

744 bpBioBricks 2010

<partinfo>BBa_K165018</partinfo>

NLS-ADH1 terminator (Silver Standard 5' and 3')

08/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq partially confirmed (MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.

253 bpBioBricks 2010

<partinfo>BBa_K165037</partinfo>

tef2 promoter

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (MIT).

403 bpBioBricks 2010

<partinfo>BBa_P1004</partinfo>

Chloramphenicol resistence
cassette

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A1</partinfo>

Seq ok (MIT 2009); length ok.

769 bpBioBricks 2010

<partinfo>BBa_J61001</partinfo>

R6K origin

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (MIT).

406 bpBioBricks 2010

<partinfo>BBa_K081008</partinfo>

RBS-luxI

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (MIT); length ok.

664 bpBioBricks 2010

<partinfo>BBa_K125500</partinfo>

GFP fusion brick (Silver Standard 5', Knight Standard 3')

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq partially confirmed (MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.

718 bpBioBricks 2010

<partinfo>BBa_K208001</partinfo>
(stab-1)

phasin phaP
(Silver Standard 5' and 3')

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

Seq ok (BMR); length ok.

579 bpBioBricks 2010

<partinfo>BBa_K208001</partinfo>
(stab-2)

phasin phaP
(Silver Standard 5' and 3')

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

Seq ok (BMR); length ok.

579 bpBioBricks 2010

<partinfo>BBa_J72007</partinfo>

BamHI methyltransferase encoding CRIM plasmid

01/07/2010

E. coli ???

HC Cm resistance

No plasmid in the clone.
For more informations click here.

BioBricks 2010

<partinfo>BBa_J72008</partinfo>

phi80 integration helper plasmid pInt80-649

01/07/2010

E. coli ???

Plasmid (Amp 50 ug/ml resistance)

Length ok.

6335 bpBioBricks 2010

<partinfo>BBa_J72013</partinfo>

BglII methyltransferase encoding CRIM plasmid

01/07/2010

E. coli ???

HC Cm resistance

No plasmid in the clone.
For more informations click here.

BioBricks 2010

<partinfo>BBa_K105012</partinfo>

10 aa flexible protein domain linker

06/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

-

30 bp BioBricks 2010

<partinfo>BBa_J13002</partinfo>

p(tetR)+RBS34

16/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

-

74 bp BioBricks 2010

<partinfo>BBa_I13522</partinfo>

Untagged GFP behind a constitutive promoter

16/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

-

937 bp BioBricks 2010
Name Ligation Creation date Strain Vector Quality control Length Description

I0
(it was I0-2)

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR); length ok.

854 bp GFP silver (GFPs)

I1
(it was I1-2)

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq ok (BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok.

1029 bp

I2
(it was I2-1)

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR); Cm resistance ok; length ok.

906 bp

I3
(it was I3-1)

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok.

801 bp

I4
(it was I4-2)

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR); length ok.

1439 bp

I5
(it was I5-1)

I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X)

18/06/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Seq ok (BMR); Cm resistance ok; length ok.

1320 bp

I6
(it was I6-2)

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok.

2754 bp

I7
(it was I7-3)

 <partinfo>BBa_J23118</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

2795 bp

I8
(it was I8-5)

<partinfo>BBa_J23110</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

2795 bp

I9
(it was I9-1)

<partinfo>BBa_J23114</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

2795 bp

I10
(it was I10-1)

<partinfo>BBa_J23116</partinfo> (E-S)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

2795 bp

I12
(it was I12-2)

<partinfo>BBa_J23105</partinfo> (S-P)
+
I6 (X-P)

16/07/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

2795 bp

I14
(it was I14-1)

<partinfo>BBa_J23118</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp

RING

I5 (X-S) on itself:
Cm resistance (<partinfo>BBa_P1004</partinfo>) + TT (<partinfo>BBa_B0015</partinfo>) + R6K ori (<partinfo>BBa_J61001</partinfo>)

16/07/2010

E. coli DH5-alpha

Plasmid (Cm resistance, conditional thermosensitive ORI; works in PIR strains)

Tested here.

1328 bp

I15
(it was I15-1)

<partinfo>BBa_J23110</partinfo> (S-P)
+
I3 (X-P)

23/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

842 bp

I16
(it was I16-1)

<partinfo>BBa_J23116</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp

I17
(it was I17-1)

<partinfo>BBa_J23101</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp

I18
(it was I18-1)

<partinfo>BBa_J23105</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp

I19
(it was I19-1)

<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp

I20
(it was I20M-1)

Pha10S-1 (X-S) (infos here)
+
<partinfo>pSB1AK3</partinfo> (X-S)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

576 bp

Pha10S

I21
(it was I21-4)

PhaSS-1 (X-S) (infos here)
+
<partinfo>pSB1A3</partinfo> (X-S)

10/08/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq ok (BMR), length ok.

576 bp

PhaSS

I22
(it was I22-1)

<partinfo>BBa_K081009</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

10/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

825 bp

I23
(it was I23-1)

<partinfo>BBa_R0062</partinfo> (S-P)
+
I3 (X-P)

10/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

725 bp

I24
(it was I24-1)

<partinfo>BBa_R0062</partinfo> (S-P)
+
I22 (X-P)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

886 bp

I26
(it was I26-2)

<partinfo>BBa_K105012</partinfo> (S-P)
+
I21 (X-P)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

612 bp

linker+PhaS

I29
(it was I29-1)

<partinfo>BBa_T9002</partinfo> (S-P)
+
I23 (X-P)

31/08/2010

E. coli TOP10

<partinfo>pSB1A3</partinfo>

Length ok.

2678 bp

I30
(it was ??)

<partinfo>BBa_T9002</partinfo> (S-P)
+
I24 (X-P)

E. coli TOP10

<partinfo>pSB1A3</partinfo>

2839 bp

I31
(it was I31-1)

I20 (E-S)
+
<partinfo>BBa_K105012</partinfo> (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

612 bp

Pha10S+linker

I32
(it was I32-2)

I20 (E-S)
+
I21 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq ok (BMR), length ok.

1158 bp

Pha10S+PhaSS

I33
(it was I33-1)

I20 (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1436 bp

Pha10S+GFPs

I34
(it was I34-1)

I21 (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1436 bp

PhaSS+GFPs

I35
(it was I35-1)

I21 (E-S)
+
<partinfo>BBa_K105012</partinfo> (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

612 bp

PhaSS+linker

I36
(it was I36-7)

I21 (E-S)
+
I21 (E-X)

24/08/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Length ok.

1158 bp

PhaSS+PhaSS

I37
(it was I37-1)

INTEIN (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1367 bp

INTEIN+GFPs

I38
(it was I38-3)

<partinfo>BBa_F2620</partinfo> (E-S)
+
<partinfo>BBa_B0034</partinfo> (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

1079 bp

HSL inducible system

I39
(it was I39-5)

<partinfo>BBa_J13002</partinfo> (S-P)
+
I20 (X-P)

24/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

652 bp

pTet+Pha10S

I40
(it was I40-1)

I26 (E-S)
+
INTEIN (E-X)

25/08/2010

E. coli DH5-alpha

pMA (HC Amp resistance)

Length ok.

1125 bp

linker+PhaSS+INTEIN

I41
(it was I41-1)

I26 (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1472 bp

I42
(it was I42-3)

CREAM (E-P)
+
<partinfo>BBa_K173001</partinfo> (E-P)

23/08/2010

E. coli BW23474

CREAM

Length ok.

3068 bp

I43

(it was I43-2)

CREAM (E-P)
+
<partinfo>BBa_J23101</partinfo> (E-P)

23/08/2010

E. coli BW23474

CREAM

Length ok.

3026 bp

I44
(it was I44-2)

CREAM (E-P)
+
<partinfo>BBa_I52002</partinfo> (E-P)

15/09/2010

E. coli DB3.1

CREAM

Seq ok (BMR).

3239 bp

I45
FAILED

CREAM (HindIII) on itself

23/08/2010

E. coli BW23474

CREAM

2149 bp

I47
(it was I47-13)

<partinfo>BBa_J13002</partinfo> (S-P) + I33 (X-P)

02/09/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

1517 bp

pTet+Pha10S+GFPs

I48
(it was I48-1)

I39 (S-P) + I34 (X-P)

31/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

2094 bp

pTet+Pha10S+PhaSS+GFPs

I49
(it was I49-1)

I39 (S-P) + I41 (X-P)

31/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

2130 bp

pTet+Pha10S+linker+PhaSS+GFPs

I50
FAILED (no trasformants on plate)

I44 (E-H) on itself

28/08/2010

E. coli DB3.1

CREAM

3239 bp

I51
(it was I51-3)

CREAM (E-P)
+
<partinfo>BBa_I52002</partinfo> (E-P)

03/09/2010

E. coli DB3.1

CREAM

3239 bp

I52
(it was I52-c)

I35 (S-P)
+
I37 (X-P)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

1985 bp

PhaSS+linker+INTEIN+GFPs

I53
(it was I53-c)

I32 (E-S)
+
I37 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

2531 bp

Pha10S+PhaSS+INTEIN+GFPs

I54
(it was I54-a)

I36 (E-S)
+
I37 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

2531 bp

PhaSS+PhaSS+INTEIN+GFPs

I55
(it was I55-X)

I38 (E-S)
+
I20 (E-X)

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

1661 bp

<partinfo>BBa_F2620</partinfo>+Pha10S

I56
(it was I56-a)

I26 (E-S)
+
I41 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

2090 bp

linker+PhaSS+linker+PhaSS+GFPs

I57
(it was I57-a)

I40 (E-S)
+
I0 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

1985 bp

linker+PhaSS+GFPs

I58
(it was I58-c)

I38 (E-S)
+
I31 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

1697 bp

<partinfo>BBa_F2620</partinfo>+Pha10S+linker

I59
(it was I59-b)

I21 (E-S)
+
I34 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

2018 bp

PhaSS+PhaSS+GFPs

I60
(it was I60-a)

I20 (S-P)
+
I26 (X-P)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

1194 bp

Pha10S+linker+PhaSS

I61
(it was I61-a)

I31 (E-S)
+
I37 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

1985 bp

Pha10S+linker+INTEIN+GFPs

I62
(it was I62-E)

I39 (S-P)
+
I59 (X-P)

27/09/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Length ok.

2678 bp

pTet+Pha10S+PhaSS+PhaSS+GFPs

I63
(it was I63-1)

I38 (E-S)
+
I61 (E-X)

24/09/2010

E. coli TOP10

<partinfo>pSB1AK3</partinfo>

Length ok.

3070 bp

<partinfo>BBA_F2620</partinfo>+Pha10S+linker+INTEIN+GFPs

I64
(it was I64-2)

I58 (E-S)
+
I52 (E-X)

24/09/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Length ok.

3688 bp

<partinfo>BBA_F2620</partinfo>+Pha10S+linker+PhaSS+linker+INTEIN+GFPs

I65
(it was I65-3)

I39 (S-P)
+
I56 (X-P)

24/09/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Length ok.

2748 bp

pTet+Pha10S+linker+PhaSS+linker+PhaSS+GFPs

I66
(it was I66-1)

I60 (E-S)
+
I57 (E-X)

24/09/2010

E. coli TOP10

<partinfo>pSB1AK3</partinfo>

Length ok.

3185 bp

Pha10S+linker+PhaSS+linker+PhaSS+INTEIN+GFPs

INTEIN

Intein

13/08/2010

E. coli TOP10

pMA
(HC Amp resistance)

Mr.Gene DNA synthesis

507 bp

YEAST
MR.G

YEAST fragment

15/09/2010

E. coli DH5-alpha

pMA
(HC Amp resistance)

Mr.Gene DNA synthesis

1695 bp

CRIM
MR.G

CREAM fragment

13/08/2010

E. coli TOP10

pMK-RQ
(HC Kan resistance)

Mr.Gene DNA synthesis

829 bp

CREAM
(it was MyCRIM)

CRIM
MR.G (A-A)
+
I5 (S-X)

21/08/2010

E. coli BW23474

CREAM

Length ok.

2149 bp

I7-4C5
(it was I7-4C5-2)

I7 (E-P)
+
<partinfo>pSB4C5</partinfo>(E-P)

16/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

2795 bp

I8-4C5
(it was I8-4C5-2)

I8 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

16/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

2795 bp

I10-4C5
(it was I10-4C5-1)

I10 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

16/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

2795 bp

I12-4C5
(it was I12-4C5-1)

I12 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

2795 bp

I14-4C5
(it was I14-4C5-2)

I14 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

23/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

I15-4C5
(it was I15-4C5-2)

I15 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

13/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

I16-4C5
(it was I16-4C5-1)

I16 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

23/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

I17-4C5
(it was I17-4C5-1)

I17 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

I18-4C5
(it was I18-4C5-1)

I18 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

I19-4C5
(it was I19-4C5-1)

I19 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

I27
(it was ??)

I23 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

??

E. coli TOP10

<partinfo>pSB4C5</partinfo>

725 bp

I28
(it was ??)

I24 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

??

E. coli TOP10

<partinfo>pSB4C5</partinfo>

886 bp

BBa_J23100
_4C5
(it was <partinfo>BBa_J23100</partinfo>
_4C5-1)

<partinfo>BBa_J23100</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

904 bp

BBa_J23101
_4C5
(it was <partinfo>BBa_J23101</partinfo>
_4C5-1)

<partinfo>BBa_J23101</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

904 bp

BBa_J23105
_4C5
(it was <partinfo>BBa_J23105</partinfo>
_4C5-1)

<partinfo>BBa_J23105</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

BBa_J23106
_4C5
(it was <partinfo>BBa_J23106</partinfo>
_4C5-1)

<partinfo>BBa_J23106</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

BBa_J23110
_4C5
(it was <partinfo>BBa_J23110</partinfo>
_4C5-1)

<partinfo>BBa_J23110</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

BBa_J23114
_4C5
(it was <partinfo>BBa_J23114</partinfo>
_4C5-1)

<partinfo>BBa_J23114</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

BBa_J23116
_4C5
(it was <partinfo>BBa_J23116</partinfo>
_4C5-1)

<partinfo>BBa_J23116</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

30/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

BBa_J23118
_4C5
(it was <partinfo>BBa_J23118</partinfo>
_4C5-1)

<partinfo>BBa_J23118</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

30/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

904 bp

pSB1C3_RFP

<partinfo>BBa_J04450</partinfo>
in <partinfo>pSB1C3</partinfo>.

??

E. coli DH5alpha

<partinfo>pSB1C3</partinfo>

1069 bp

2009 Distribution
pSB1C3_ccdB

<partinfo>BBa_P1010</partinfo>
in <partinfo>pSB1C3</partinfo>.

??

E. coli DH5alpha

<partinfo>pSB1C3</partinfo>

675 bp

2009 Distribution
Strain name Description Arrival date Infos Resistance Quality control Notes Store

MG1655(Seq)
(MG)

E. coli wild type strain fully sequenced

28/06/2010

link

- Genome fully sequenced Strains 2010

MC1061
(MC)

E. coli strain

28/06/2010

link

- Strain used by JC Anderson et al. for integration Strains 2010

BW25141
(BW1)

E. coli pir+ strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K LC pir Strains 2010

BW25142
(BW2)

E. coli pir116 strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K HC pir Strains 2010

BW23473

E. coli pir+ strain

19/07/2010

link

- LC pir Strains 2010

BW23474

E. coli pir116 strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K HC pir Strains 2010

BW5328/pAH123

E. coli strain

28/06/2010

link

Amp 50 ug/ml Plasmid length ok (after digestion with SpeI).

Contains pAH123 HELPER plasmid carrying IntPhi80, low copy Amp, ori-ts 30°C

Strains 2010

BT340

E. coli strain

28/06/2010

link

Amp 100 ug/ml and Cm 34 ug/ml Plasmid relative molecular weight ok (after gel run of the undigested plasmid)

Contains pCP20 helper with yeast FRT recombinase, Amp, Cm, ori-ts 30°C

Strains 2010

DSM15372

E. coli DMS15372 strain

08/07/2010

link

Amp 100 ug/ml Length ok (after digestion with EcoRI)

Harbours the pBHR68 plasmid (contains an IPTG-inducible phaCAB operon)

Strains 2010
Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Freezer"