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UTDallas/23 September 2010
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(New page: *Prepared glycerol stock of the transformed ligated parts, in case they are correct. *Miniprepped the transformed ligated parts, and then digested it. *We also digested another part using ...)
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(New page: *Prepared glycerol stock of the transformed ligated parts, in case they are correct. *Miniprepped the transformed ligated parts, and then digested it. *We also digested another part using ...)
Newer edit →
Revision as of 11:48, 28 September 2010
- Prepared glycerol stock of the transformed ligated parts, in case they are correct.
- Miniprepped the transformed ligated parts, and then digested it.
- We also digested another part using all the enzymes to test the enzymes, because we have gotten poor digestions in the past.
- We ran all the digestions on two gels. (pictures are attached)
- The first gel is the digested parts. They are not correct, therefore are ligation did not work. We will try again tomorrow.
- The second gel is the enzymes. The resulting gel was strange, especially the two middle results. This did not tell us much.
