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Team:Lethbridge/Notebook/Lab Work/June
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<b>Objective:</b> Isolate plasmid DNA (BBa_J33204) from DH5α cells and confirm results.<br> | <b>Objective:</b> Isolate plasmid DNA (BBa_J33204) from DH5α cells and confirm results.<br> | ||
| - | <b>Method:</b> "Mini-prep" the plasmid DNA using | + | <b>Method:</b> "Mini-prep" the plasmid DNA using [[Team:Lethbridge/Notebook/Protocols|boiling lysis miniprep]]. Then restrict the DNA once and run on a 1% agarose gel (TAE). <br> |
===June 3/2010=== | ===June 3/2010=== | ||
<b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br> | <b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br> | ||
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June 2010
June 1/2010
JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in working plasmids box.
June 2/2010
(In Lab: JV)
Objective: Isolate plasmid DNA (BBa_J33204) from DH5α cells and confirm results.
Method: "Mini-prep" the plasmid DNA using boiling lysis miniprep. Then restrict the DNA once and run on a 1% agarose gel (TAE).
June 3/2010
Objective: Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.
