Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/06
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===4:Ligation=== | ===4:Ligation=== |
Revision as of 06:48, 28 September 2010
Contents |
1:PCR
<Member>
Hitomi,nito
<Sample>
- 1(BBa_K208017)
- 2(BBa_JM109)
- 5(BBa_K208017)
<Protocol>
See Protocol 2
Tubes(Temperature in annealing)
- 1(BBa_K208017) 71.5,72.0,72.5℃
- 2(BBa_JM109) 71.0,71.5,72.0℃
- 5(BBa_K208017) 70.0,70.5,71.0℃
2:DNA extraction
<Member>
Hitomi
<Protocol>
See Protocol 4
3:Electrophoresis
<Samlpe>
PCR products.
- 1
- 2
- 5
- 7
- 8
<Protocol>
See Protocol 8
<Result>
4:Ligation
<Membber>
nito
<Sample>
- 7
- 8
<Protocol>
See Protocol 3