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Team:LMU-Munich/Notebook/Protocols/24 Colony PCR
From 2010.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
|2.5µl | |2.5µl | ||
| - | |- | + | |- |
| - | | | + | |primer forward (BioBrick) |
|0.375µl | |0.375µl | ||
|- | |- | ||
| - | | | + | |primer revers (BioBrick) |
|0.375µl | |0.375µl | ||
Latest revision as of 12:47, 27 September 2010
Colony PCR
Component
Volume
PCR Master Mix
2.5µl
primer forward (BioBrick)
0.375µl
primer revers (BioBrick)
0.375µl
nuclease free water to final volume of
5µl
Guidelines for Amplification by PCR
