Team:LMU-Munich/Notebook/Protocols/24 Colony PCR


Colony PCR

Component Volume
PCR Master Mix 2.5µl
primer forward (BioBrick) 0.375µl
primer revers (BioBrick) 0.375µl
nuclease free water to final volume of 5µl

Guidelines for Amplification by PCR

A. Denaturation:
initial: 94°C 5'
subsequent: 30
B. Annealing:
53°C 30
C. Extension
72°C 1'30
final: 8'
D. Refrigeration
E.Cycle Number
30 cycles