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Team:Brown/Notebook/September18
From 2010.igem.org
(Difference between revisions)
(New page: {{:Team:Brown/templates/header}} ==Saturday, September 17, 2010== ===Retrieval of yesterday's transformation=== Retrieved transforms 20h after incubation *Colonies grew to high density, s...) |
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Will redo transformation with 2ul of ligation product from 9/16 | Will redo transformation with 2ul of ligation product from 9/16 | ||
| - | *Add | + | *Add the following to 2ul of ligation product: |
**50ul 20mM xGal | **50ul 20mM xGal | ||
**2ul 1M IPTG | **2ul 1M IPTG | ||
Revision as of 01:20, 26 September 2010
Saturday, September 17, 2010
Retrieval of yesterday's transformation
Retrieved transforms 20h after incubation
- Colonies grew to high density, some secondary colony growth?
- No blue/white selection used to screen for insertion of Tat-L into pBS
Will redo transformation with 2ul of ligation product from 9/16
- Add the following to 2ul of ligation product:
- 50ul 20mM xGal
- 2ul 1M IPTG
- 100ul LB
Incubated at 3:30PM, plated all 300ul of mixture
To be out at 12pm, 9/19
