Team:UNIPV-Pavia/Calendar/September/settimana4

From 2010.igem.org

(Difference between revisions)
(September, 21st)
(September, 21st)
Line 54: Line 54:
* I56 : 159,6 ng/ul
* I56 : 159,6 ng/ul
* I57 : 103 ng/ul
* I57 : 103 ng/ul
-
* 161 : 147,1 ng/ul
+
For I61 the DNA is already available quantified 147,1 ng/ul.
-
I61
+
 
 +
After 3 hour digestion was performed:
 +
 

Revision as of 13:09, 24 September 2010


SEPTEMBER: WEEK 4



September, 20th

Screening PCR on 6 colonies picked from I55 was performed. The results were the follow:

I55.

Inoculum of I52, I53, I55, I56 and I57 in LB+Amp for MiniPrep of the following day

September, 21st

Miniprep and Nanodrop quantification for:

  • I52 : 92,8 ng/ul
  • I53 : 118,5 ng/ul
  • I55 : 121,1 ng/ul
  • I56 : 159,6 ng/ul
  • I57 : 103 ng/ul

For I61 the DNA is already available quantified 147,1 ng/ul.

After 3 hour digestion was performed:


Tecan Test was performed on prepared samples, after the usual protocol (dilution, medium change and dilution 1:1000).

September, 22nd

September, 23rd

September, 24th

September, 25th