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Team:Newcastle/Meetings/4 June 2010
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* '''Group 1 starts 14th June''': SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis) | * '''Group 1 starts 14th June''': SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis) | ||
| - | * '''Group 2 starts 21st June''': JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; | + | * '''Group 2 starts 21st June''': JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Spider silk and filamentous cells) |
* All team members working on BioBrick 1 (CaCO3/Urease). | * All team members working on BioBrick 1 (CaCO3/Urease). | ||
Revision as of 15:16, 9 June 2010
| Informal Meeting 04.06.2010 |
| Urease tutorial-recap |
| Accessory genes and nickel incorporation necessary more complicated than we thought. |
| Software engineering cycle |
| Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements |
| Specification more detail on the requirements- literature |
| Urease calcium carbonate, optimise for Bacillis |
| Increase expression- what we thought! |
| Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper |
| Accessory genes required! |
| Look over papers from tutorial |
| Sporosarcina pasteurii |
| Biobrick site symbols |
| Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers |
| pSpac |
| ncbi – find the genome – can see the genes |
| Implementation using sequencher |
| Find the sequence of part |
| Assemble parts |
| Biobrick friendly restriction sites |
| Genbank/embl file format |
| Implementation altered by cloning strategy |
| Biobricks RFC- request for comments |
| http://Bbf.openware.org/RFC.html -duplo and lego don’t fit together |
| AP Rachel: video for lego pipette:http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded |
| 1st lab week Others modelling Filamentous and spidersilk killswitch |
| RC |
| DY |
| AK |
| (HS) |
| 2nd lab week Others Modelling glue and lysis quorum sensing |
| JS |
| RB |
| SW |
| PH |
| (YE) |
| Urease both
LAB TRAINING GROUPS:
|
