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Team:Edinburgh/Bacterial/Green light producer
From 2010.igem.org
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<li><a href="https://2010.igem.org/Team:Edinburgh/Notebook" class="dir">lab notes </a> | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook" class="dir">lab notes </a> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">collaboration</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Collaboration">collaboration</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/BRIDGE">BRIDGE</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/BRIDGE">BRIDGE</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Red_light_producer">red light</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Red_light_producer">red light</a></li> | ||
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<p>The green luciferase will require the combination of luxAB with the production of another protein: YFP from ''Vibrio fischeri''. This will shift the wavelength of the light towards the yellow spectrum, making it the correct colour to activate our green light sensor. Mexico UNAM-Genomics have had this DNA sequence synthesised and sending it to us for characterisation and combination with luxAB. We also expect Mexico to BioBrick it as part of their submission to the registry.</p> | <p>The green luciferase will require the combination of luxAB with the production of another protein: YFP from ''Vibrio fischeri''. This will shift the wavelength of the light towards the yellow spectrum, making it the correct colour to activate our green light sensor. Mexico UNAM-Genomics have had this DNA sequence synthesised and sending it to us for characterisation and combination with luxAB. We also expect Mexico to BioBrick it as part of their submission to the registry.</p> | ||
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| + | <a name="Strategy" id="Strategy"></a><h2>Strategy</h2> | ||
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<a name="Problems" id="Problems"></a><h2>Problems</h2> | <a name="Problems" id="Problems"></a><h2>Problems</h2> | ||
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| - | You can't have problems with a piece of DNA you've never met. | + | <p>You can't have problems with a piece of DNA you've never met.</p> |
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Revision as of 12:22, 22 September 2010
Overview: The green light producer
The green luciferase will require the combination of luxAB with the production of another protein: YFP from ''Vibrio fischeri''. This will shift the wavelength of the light towards the yellow spectrum, making it the correct colour to activate our green light sensor. Mexico UNAM-Genomics have had this DNA sequence synthesised and sending it to us for characterisation and combination with luxAB. We also expect Mexico to BioBrick it as part of their submission to the registry.
Strategy
***
Problems
You can't have problems with a piece of DNA you've never met.
Biobricks
***
References
***
