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Revision as of 13:52, 12 September 2010 (view source) Mizuki (Talk | contribs) (→4:Ligation) ← Older edit		Revision as of 10:08, 22 September 2010 (view source) Mariko (Talk | contribs) (→3:Electrophoresis) Newer edit →
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	===4:Ligation===		===4:Ligation===

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Revision as of 10:08, 22 September 2010

Contents 1 2010/09/06 1.1 1:PCR 1.2 2:Purification of PCR 1.3 3:Electrophoresis 1.4 4:Ligation

2010/09/06

1:PCR

<Member>

Hitomi,nito

<Sample>

• 1(BBa_K208017)
• 2(BBa_JM109)
• 5(BBa_K208017)

<Protocol>

See Protocol2

(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)

Tubes(Temperature in annealing)

• 1(BBa_K208017) 71.5,72.0,72.5℃
• 2(BBa_JM109) 71.0,71.5,72.0℃
• 5(BBa_K208017) 70.0,70.5,71.0℃

2:Purification of PCR

<Member>

Hitomi

<Protocol>

See Protocol

3:Electrophoresis

<Samlpe>

PCR products.

• 1
• 2
• 5
• 7
• 8

<Protocol>

See Protocol8

(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)

<Result>

File:Matsuura 2010-09-06 16hr 49min.tif

4:Ligation

<Membber>

nito

<Sample>

• 7
• 8

<Protocol>

See Protocol10