"
Team:Edinburgh/BioBricks
From 2010.igem.org
(Difference between revisions)
| Line 123: | Line 123: | ||
<br> | <br> | ||
| - | <p>For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing ''cat'' (chloramphenicol resistance) and ''sacB'' (prevents growth on sucrose). | + | <p>For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing ''cat'' (chloramphenicol resistance) and ''sacB'' (prevents growth on sucrose).<br> |
Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on ''sacB''. | Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on ''sacB''. | ||
| + | <br><br> | ||
In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed ''E. coli'' no longer smells!</p> | In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed ''E. coli'' no longer smells!</p> | ||
Revision as of 11:23, 20 September 2010
BioBricks: Genomic BRIDGEs
For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing ''cat'' (chloramphenicol resistance) and ''sacB'' (prevents growth on sucrose).
Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on ''sacB''.
In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed ''E. coli'' no longer smells!
