Team:Newcastle/Glycerol stocks
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(New page: =Making glycerol stocks= ==Materials== * 1.5ml microcentrifuge tubes * 2ml collection tube * QIAquick columns * Buffer PB * Buffer EB * Buffer PE * DNA mixture from PCR ==Protocol== # ...)
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(New page: =Making glycerol stocks= ==Materials== * 1.5ml microcentrifuge tubes * 2ml collection tube * QIAquick columns * Buffer PB * Buffer EB * Buffer PE * DNA mixture from PCR ==Protocol== # ...)
Newer edit →
Revision as of 10:10, 19 September 2010
Making glycerol stocks
Materials
- 1.5ml microcentrifuge tubes
- 2ml collection tube
- QIAquick columns
- Buffer PB
- Buffer EB
- Buffer PE
- DNA mixture from PCR
Protocol
- Inoculate 5ml THY Broth with a single colony and incubate, stationary at 37°C for 8 hours.
- 0.85ml
Go back to our Protocol List
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