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Team:UT-Tokyo/Sudoku notebook 2
From 2010.igem.org
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==== 8/4 ==== | ==== 8/4 ==== | ||
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'''Inoculation''' | '''Inoculation''' | ||
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iii 1,5,11,7,8,4Ⅱ EXcut (7/29 Miniprep) | iii 1,5,11,7,8,4Ⅱ EXcut (7/29 Miniprep) | ||
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| + | '''Gel extract''' | ||
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| + | i 1-99234 8.8ng/uL | ||
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| + | ii 1-9234 7.1ng/uL | ||
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| + | iii 1-5-11-7-8-4 18.0ng/uL | ||
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| - | ''' | + | '''Miniprep''' |
| - | + | 12-i 58.7ng/uL | |
| - | + | 12-ii 55.9ng/uL | |
| - | + | 13-i 48.7ng/uL | |
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| + | 13-ii 48.5ng/uL | ||
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| + | (8/4 Inoculation) | ||
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sudoku (1234) 6.6ng/ul | sudoku (1234) 6.6ng/ul | ||
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| + | '''Ligation''' | ||
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| + | 0(1234)&iii | ||
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(8/4 & 8/5) | (8/4 & 8/5) | ||
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==== 8/9 ==== | ==== 8/9 ==== | ||
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| + | '''''' | ||
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| + | '''''' | ||
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| + | '''''' | ||
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==== 7/5 ==== | ==== 7/5 ==== | ||
==== 7/5 ==== | ==== 7/5 ==== | ||
Revision as of 02:32, 19 September 2010
Sudoku
Abstract Construct Experiment Notebook Result
Notebook
Pre-experiment
AUGUST!!!!!
AUGUST!!!!!
AUGUST!!!!!
AUGUST!!!!!
AUGUST!!!!!
Make construct
8/4
Inoculation
sudoku 12-i & 12-ii
sudoku 13-i & 13-ii
(8/3 Transformation)
Enzyme digestion
i 1-99234Ⅰ EScut (7/24 Miniprep & Sequencing)
ii 1-9234Ⅰ EScut (7/21 Miniprep / 7/24 Sequencing)
iii 1,5,11,7,8,4Ⅱ EXcut (7/29 Miniprep)
Gel extract
i 1-99234 8.8ng/uL
ii 1-9234 7.1ng/uL
iii 1-5-11-7-8-4 18.0ng/uL
Ligation
i-iii i:6ul iii:6ul ligationmix 12ul
ii-iii ii:6ul iii:4ul ligationmix 10ul
(8/4 Miniprep)
Miniprep
12-i 58.7ng/uL
12-ii 55.9ng/uL
13-i 48.7ng/uL
13-ii 48.5ng/uL
(8/4 Inoculation)
8/5
Enzyme digestion
1234（107.2ng/uL）(8/4 gel extract)
Gel extract
sudoku (1234) 6.6ng/ul
Ligation
0(1234)&iii
Transformation
i-iii i & iii
ii-iii ii & iii
0(1234) & iii
(8/4 & 8/5)
8/6
Inoculation
0-iii,(i-iii),ii-iii (8/5 transformation)
transformation
i-iii (8/4 ligation)
8/9
'
'
'
'
7/5
7/5
7/5
Ligation
2-3 (7/3 enzyme cut) & 4 / 11 (7/3 enzyme cut) & 7
2-3 : 37.5 ng/uL → 5 uL & 4 : 5.2 ng/uL → 5 uL
11 : 4.2 ng/uL → 7.5 uL & 7 : Unknown → 2.5 uL
Transformation
2-3-4 plasmid (7/5 ligation) / 11-7 plasmid (7/5 ligation)
Check test
Main construct
Research of SP6 RNApol cDNA and F plasmid
Convert lox71(rev) to lox66(rev)
Make Hin(rev)
Revise MS2 gene
Make location(rev)-rbs(rev)-location(rev)
