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Team:UNIPV-Pavia/Calendar/September
From 2010.igem.org
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<tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana1 #September, 1st|September, 1st]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana1 #September, 1st|September, 1st]]</td><td align="left"> | ||
| - | 71° Bio-Lab - | + | 71° Bio-Lab - PCR from colony for I47 ligation.Tecan Test was performed on prepared samples. |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana1 #September, 2nd|September, 2nd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana1 #September, 2nd|September, 2nd]]</td><td align="left"> | ||
| - | 72° Bio-Lab - | + | 72° Bio-Lab - Again PCR from colony for I47 ligation: I47-10..26.Tecan Test was performed on prepared samples. |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana1 #September, 3rd|September, 3rd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana1 #September, 3rd|September, 3rd]]</td><td align="left"> | ||
| - | 73° Bio-Lab - | + | 73° Bio-Lab - Today we got our yeast strains. |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana1 #September, 4th|September, 4th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana1 #September, 4th|September, 4th]]</td><td align="left"> | ||
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<tr><th align="center" width="12%" colspan="2"><b>SEPTEMBER: WEEK 2</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>SEPTEMBER: WEEK 2</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 6th|September, 6th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 6th|September, 6th]]</td><td align="left"> | ||
| - | 76° Bio-Lab - | + | 76° Bio-Lab - Inoculum of I47, I48, I49 into 5 ml LB+Amp for TECAN experiment. |
| + | |||
| + | Inoculum of I44-2 and I51-3 into 5 ml LB+Cm34 to evaluate MiniPrep yields. | ||
| + | |||
| + | Inoculum of I44-2 and I51-3 into 5 ml LB+Cm12,5 to evaluate MiniPrep yields and for sequencing. | ||
| + | |||
| + | Inoculum for MiniPrep and sequencing of: <partinfo>BBa_J23100</partinfo>_4C5, <partinfo>BBa_J23101</partinfo>_4C5, <partinfo>BBa_J23105</partinfo>_4C5, <partinfo>BBa_J23106</partinfo>_4C5, <partinfo>BBa_J23110</partinfo>_4C5, <partinfo>BBa_J23114</partinfo>_4C5, <partinfo>BBa_J23116</partinfo>_4C5, <partinfo>BBa_J23118</partinfo>_4C5, I7-A and I8-5-D. | ||
| + | |||
| + | Inoculum of cultures for TECAN test on self-inducible promoters. | ||
| + | |||
| + | Glycerol stocks of MG43, MC42, MC43. Static TECAN test (absorbance, green fluorescence and red fluorescence) of these cultures. | ||
| + | |||
| + | Liquid PCR was performed on MG43, MC42, MC43 and MC1061. | ||
| + | MiniPreps of I44-1:5 and I51-1:5 were performed. | ||
| + | |||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 7th|September, 7th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 7th|September, 7th]]</td><td align="left"> | ||
| - | 77° Bio-Lab - <br> 13° Meeting - Updating about Bio-Lab activity. | + | 77° Bio-Lab -MiniPreps of <partinfo>BBa_J23100_4C5</partinfo>, <partinfo>BBa_J23101_4C5</partinfo>, <partinfo>BBa_J23105_4C5</partinfo>, <partinfo>BBa_J23106_4C5</partinfo>, <partinfo>BBa_J23110_4C5</partinfo>, <partinfo>BBa_J23114_4C5</partinfo>, <partinfo>BBa_J23116_4C5</partinfo>, <partinfo>BBa_J23118_4C5</partinfo>, I7-A, I8-5-D, I44-2, I51-3, I44-2, I51-3 and I47 were performed. |
| + | Trasformation of I42 and I43. Integration protocol's first day were performed. | ||
| + | <br> | ||
| + | 13° Meeting - Updating about Bio-Lab activity. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 8th|September, 8th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 8th|September, 8th]]</td><td align="left"> | ||
| - | 78° Bio-Lab | + | 78° Bio-Lab - Inoculum of: I31, I32, I35, I36, I37, I38, I40, I41 and I20 for second step of ligation of phasins/intein. |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 9th|September, 9th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 9th|September, 9th]]</td><td align="left"> | ||
| - | 79° Bio-Lab - | + | 79° Bio-Lab - Minipresp of I31, I32, I35, I36, I37, I38, I40, I41 and I20 were performed. |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 10th|September, 10th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 10th|September, 10th]]</td><td align="left"> | ||
| - | 80° Bio-Lab - | + | 80° Bio-Lab - Screening digestion of I44-2 and I51-3. |
| + | |||
| + | Gel run/cut for samples of previous day (I31, I32, I35, I36, I37, I38, I40, I41 and I20). | ||
| + | Digestions were ligated in order to create: I53, I54, I55, I56, I57, I58. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 11th|September, 11th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana2 #September, 11th|September, 11th]]</td><td align="left"> | ||
| - | 81° Bio-Lab - | + | 81° Bio-Lab - MC42 and MC43 plates were transfered. |
| + | |||
| + | I42 and I43 have been transformed in MG008 strain, plated and incubated. | ||
</td></tr> | </td></tr> | ||
<tr><th align="center" width="12%" colspan="2"><b>SEPTEMBER: WEEK 3</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>SEPTEMBER: WEEK 3</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 13th|September, 13th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 13th|September, 13th]]</td><td align="left"> | ||
| - | 82° Bio-Lab - | + | 82° Bio-Lab - Trasformation of ligations I52, I54, I55, I56, I57 and I58. into E. coli DH5-alpha. |
| + | |||
| + | Inoculum of I26, I31 and I34. | ||
| + | |||
| + | Inoculum from single colony of MG42 and MC43. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 14th|September, 14th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 14th|September, 14th]]</td><td align="left"> | ||
| - | 83° Bio-Lab - | + | 83° Bio-Lab - Agar plates of I52, I54, I55, I56, I57 and I58 were checked. |
| + | |||
| + | Miniprep and quanfification of I26, I31 and I34. After 3 hour digestion, gel run/cut of samples was done. | ||
| + | Gel exctraction and ligation was performed in order to obtain I59, I60, I61 and I53. | ||
| + | |||
| + | Inoculum of I47, I48, I49 into 5 ml LB+Amp for TECAN test of the following day. | ||
| + | |||
| + | Screening PCR on 3 colonies picked from MC42 and MC43 respectively. | ||
| + | |||
| + | Tecan Test was performed on prepared samples, after usual protocol. | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 15th|September, 15th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 15th|September, 15th]]</td><td align="left"> | ||
| - | 84° Bio-Lab | + | 84° Bio-Lab - Tecan Test was started to check GFP production by BioBricks in I47, I48, I49 cultures. |
| + | |||
| + | Transformation of I 53, I59, I60 and I61. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 16th|September, 16th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 16th|September, 16th]]</td><td align="left"> | ||
| - | 85° Bio-Lab - | + | 85° Bio-Lab - Screening through "colony PCR" for ligations I52, I53, I54, I55, I56, I57, I58, I59, I60 and I61 was performed. |
| + | |||
| + | Tecan Test was performed on prepared samples, after the usual protocol. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 17th|September, 17th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana3 #September, 17th|September, 17th]]</td><td align="left"> | ||
Revision as of 09:30, 17 September 2010
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