Team:TU Delft/13 September 2010 content
From 2010.igem.org
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Revision as of 08:07, 17 September 2010
Emulsifier
Our construct from Mr Gene has arrived! We transformed K12, Top10 and DH5alpha immediately.
Alkane degradation
In order to check if the construct of 028 (J61100-rubA4-J61100-rubR) is correct we did a digestion check.
# | BioBrick | Cut with | Buffer | BSA | Expected Length (bp) | Correct? |
0 | Smartladder (5μl) | |||||
2 | 028 | PvuI, AhdI | Buffer 2 (NEB) | BSA (1x) | 370,424,742,854,1165 | |
3 | 028 | PvuI | Buffer 2 (NEB) | BSA (1x) | 1596,1959 | |
4 | 028 | Uncut | Circulized | |||
5 | EZload ladder (5μl |