Team:Lethbridge/Notebook/Lab Work/June
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==June 2010== | ==June 2010== | ||
- | ===June 1/2010=== | + | ===June 1/2010=== |
JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in [[Team:Lethbridge/Notebook/Working_Plasmids|working plasmids box]].<br><br> | JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in [[Team:Lethbridge/Notebook/Working_Plasmids|working plasmids box]].<br><br> | ||
- | ===June 2/2010=== | + | ===June 2/2010=== |
<b>Objective:</b> Isolate plasmid DNA (BBa_J33204) from DH5α cells | <b>Objective:</b> Isolate plasmid DNA (BBa_J33204) from DH5α cells | ||
- | ===June 3/2010=== | + | ===June 3/2010=== |
<b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br> | <b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br> |
Revision as of 00:54, 5 June 2010
Contents |
June 2010
June 1/2010
JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in working plasmids box.
June 2/2010
Objective: Isolate plasmid DNA (BBa_J33204) from DH5α cells
June 3/2010
Objective: Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.