"
Team:DTU-Denmark/Wet lab
From 2010.igem.org
(Difference between revisions)
(→N-protein plasmid) |
|||
| Line 7: | Line 7: | ||
!align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Modeling|<span style="color:white;">Modeling</span>]] | !align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Modeling|<span style="color:white;">Modeling</span>]] | ||
!align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Wet_lab|Wet Lab]] | !align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Wet_lab|Wet Lab]] | ||
| - | !align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Gallery|<span style="color:white;">Gallery</span]] | + | !align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Gallery|<span style="color:white;">Gallery</span>]] |
|} | |} | ||
Revision as of 14:13, 14 September 2010
 | Home | Team | Project | Parts Submitted to the Registry | Modeling | Wet Lab | Gallery |
|---|
Anti-Terminator experimental setup
N-protein plasmid
The N protein were isolated from salmonella genomic DNA with specific designed primers. We used the natural occurring RBS site, as a High expression of N have shown non specific anti-termination effect on a global scale on the genome. #References References
References
- NC Franklin, JH Doelling - Am Soc Microbiol "Overexpression of N antitermination proteins of bacteriophages lambda, 21, and P22: loss of N protein specificity." - Journal of bacteriology, 1989
