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Team:Washington/Project/Safety/Secretion
From 2010.igem.org
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Our work on Gram-negative Therapeutics was done only on non-pathogenic strains. We used the Type VI Secretion System from ''Pseudomonas aeruginosa'' PAO1. All modifications and testing of plasmids and fosmids were in non-pathogenic E. Coli strains, namely EPI300, SW102, and BL21. All cloning and testing of Tse2 was done in the non-pathogenic E. coli strains DH5a, MG1655, and BL21. | Our work on Gram-negative Therapeutics was done only on non-pathogenic strains. We used the Type VI Secretion System from ''Pseudomonas aeruginosa'' PAO1. All modifications and testing of plasmids and fosmids were in non-pathogenic E. Coli strains, namely EPI300, SW102, and BL21. All cloning and testing of Tse2 was done in the non-pathogenic E. coli strains DH5a, MG1655, and BL21. | ||
| - | The toxin we work with, Tse2, targets only prokaryotic cells, thus posing no direct danger to humans. At no time was a working type six secretion system placed into 'E. coli' producing Tse2. Therefore, none of the strains could cause any possible ecological danger. | + | The toxin we work with, Tse2, targets only prokaryotic cells, thus posing no direct danger to humans. At no time was a working type six secretion system placed into ''E. coli'' producing Tse2. Therefore, none of the strains could cause any possible ecological danger. |
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Revision as of 00:16, 14 September 2010
Our work on Gram-negative Therapeutics was done only on non-pathogenic strains. We used the Type VI Secretion System from Pseudomonas aeruginosa PAO1. All modifications and testing of plasmids and fosmids were in non-pathogenic E. Coli strains, namely EPI300, SW102, and BL21. All cloning and testing of Tse2 was done in the non-pathogenic E. coli strains DH5a, MG1655, and BL21. The toxin we work with, Tse2, targets only prokaryotic cells, thus posing no direct danger to humans. At no time was a working type six secretion system placed into E. coli producing Tse2. Therefore, none of the strains could cause any possible ecological danger.
