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Team:Newcastle/Meetings/9 September 2010
From 2010.igem.org
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* ''yneA'' - Transforming into ''Bacillus subtilis'' 168 strains works and we have filamentous cells. However still not able to integrate ''yneA'' into Pmutin strains. Could use a plasmid that have already contain the IPTG system. For characterization, we could either do a titration with IPTG together with time lapse microscopy to measure the length of the cells. | * ''yneA'' - Transforming into ''Bacillus subtilis'' 168 strains works and we have filamentous cells. However still not able to integrate ''yneA'' into Pmutin strains. Could use a plasmid that have already contain the IPTG system. For characterization, we could either do a titration with IPTG together with time lapse microscopy to measure the length of the cells. | ||
*Arabinose promoter - Insert the promoter in front of GFP and characterize with the addition of arabinose. | *Arabinose promoter - Insert the promoter in front of GFP and characterize with the addition of arabinose. | ||
| + | *Levan glue - Able to stick sand together. Mix the glue with sand and filamentous cells and look under the microscope. Also to mix the glue with sand to produce the iGEM logo. | ||
==Concrete== | ==Concrete== | ||
Revision as of 20:42, 13 September 2010
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Contents |
Roll calls
- Apologies: Phil and Deena
- Absence: Colin Harwood and Colin Davie
Modelling
Metabolic Flux Balance Analysis - Show the judges what happen when we alter the enzyme levels to produce different outcomes.
Lab feedback
- Gibson cloning - Joining of 2 fragments together works, but could not be ligated into the vector. Need more time to perfect the technique.
- Subtilin immunity - Same as rocF
- yneA - Transforming into Bacillus subtilis 168 strains works and we have filamentous cells. However still not able to integrate yneA into Pmutin strains. Could use a plasmid that have already contain the IPTG system. For characterization, we could either do a titration with IPTG together with time lapse microscopy to measure the length of the cells.
- Arabinose promoter - Insert the promoter in front of GFP and characterize with the addition of arabinose.
- Levan glue - Able to stick sand together. Mix the glue with sand and filamentous cells and look under the microscope. Also to mix the glue with sand to produce the iGEM logo.
Concrete
- Use different bacteria to seal up the cracks.
- Preincubate the concrete in media.
Wiki
Lab book is up to date.
Visas
ESTA must be completed (if required) ASAP.
T-shirt
Upload the t-shirt files into the dropbox.
Action points
- Neil and Jen - To register the team.
- Alan - To email Jen about the IPTG plasmid and the letters for the sucrose plates.
- Harsh - To crack the concrete into smaller pieces.
- Wendy - To talk to the EM people.
Item(s) for next agenda
- Go through the presentation slides and refine. Cut down the number of slides so that it fits the 20 minutes time limit
Next meeting
- Chair: Harsh, Minutes: Younus, Computer: N/A
- Wednesday afternoon, 3pm, CBCB.
   
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