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Team:Newcastle/Meetings/9 September 2010
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(New page: {{Team:Newcastle/mainbanner}} ==Roll calls== *Apologies: Phil and Deena *Absence: Colin Harwood and Colin Davie ==Modelling== Metabolic Flux Balance Analysis - alter enzyme levels to pr...) |
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* Gibson cloning - Joining of 2 fragments together works, but could not be ligated into the vector. Need more time to perfect the technique. | * Gibson cloning - Joining of 2 fragments together works, but could not be ligated into the vector. Need more time to perfect the technique. | ||
* Subtilin immunity - Same as ''rocF'' | * Subtilin immunity - Same as ''rocF'' | ||
| - | * ''yneA'' - Transforming into ''Bacillus subtilis'' 168 strains works and we have filamentous cells. However still not able to integrate ''yneA'' into Pmutin strains. Could use a plasmid that have already contain the IPTG system. | + | * ''yneA'' - Transforming into ''Bacillus subtilis'' 168 strains works and we have filamentous cells. However still not able to integrate ''yneA'' into Pmutin strains. Could use a plasmid that have already contain the IPTG system. For characterization, we could either do a titration with IPTG together with time lapse microscopy to measure the length of the cells. |
==Concrete== | ==Concrete== | ||
Revision as of 20:31, 13 September 2010
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Contents |
Roll calls
- Apologies: Phil and Deena
- Absence: Colin Harwood and Colin Davie
Modelling
Metabolic Flux Balance Analysis - alter enzyme levels to produce different outcomes and makes complex modelling to be seen easier. We need to compare the actual lab work and the modelling to spot any changes, and if so, state the reason.
Lab feedback
- Gibson cloning - Joining of 2 fragments together works, but could not be ligated into the vector. Need more time to perfect the technique.
- Subtilin immunity - Same as rocF
- yneA - Transforming into Bacillus subtilis 168 strains works and we have filamentous cells. However still not able to integrate yneA into Pmutin strains. Could use a plasmid that have already contain the IPTG system. For characterization, we could either do a titration with IPTG together with time lapse microscopy to measure the length of the cells.
Concrete
- Three cracked concrete samples picked up. Can be taken into the lab
- Use of Electron Microscope (EM) to see the detailed cracks within the concrete
- Wendy has ordered Natto and the other strain, but have not yet arrived
Wiki
Lab book is up to date.
Visas
ESTA must be completed (if required) ASAP.
Publicity
Interview from India.
Finance
The transportation will be free, but most of the foods will need to be covered by individuals.
Action points
- Steve - Go through each point on the medal criteria list and write a couple of sentences about how we're going to tackle them
- Steve - Look into Metabolic Flux Balance Analysis and the softwares that are related to it
- Steve - Ask Collin Harwood about Bacillus sphaericus
- Harsh - Read the paper about Bacillus sphaericus
- Neil and Jen - Ask Peter Andras
- Everyone - Complete (if required) ESTA forms ASAP
Item(s) for next agenda
- Go through the presentation slides and refine. Cut down the number of slides so that it fits the 20 minutes time limit
Next meeting
- Chair: Harsh, Minutes: Younus, Computer: N/A
- Wednesday afternoon, 3pm, CBCB.
   
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