Team:SDU-Denmark/labnotes9

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(→Restriction Digest of Gel Extracted FlhDCmut and pSB1C3 and pSB1AK3)
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'''Protocol:''' [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]<Br>
'''Protocol:''' [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]<Br>
'''Notes:''' <Br>
'''Notes:''' <Br>
 +
''Restriction Digest mixtures''<Br>
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<html>
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<head>
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  <meta content="text/html; charset=ISO-8859-1"
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http-equiv="content-type">
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  <title></title>
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</head>
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<body style="width: 520px;">
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<span style="font-weight: bold;">FlhDCmut and pSB1C3
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Restriction digest</span><span style="font-style: italic;"><br>
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FlhDCmut</span><br>
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<table style="text-align: left; width: 212px;" border="1"
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cellpadding="2" cellspacing="2">
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  <tbody>
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    <tr>
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      <td style="width: 108px;">H<small><small><small><small>2<big><big><big><big>O</big></big></big></big></small></small></small></small></td>
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      <td style="text-align: right; width: 87px;">48μL</td>
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    </tr>
 +
    <tr>
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      <td style="width: 108px;"><span
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style="font-weight: bold;">EcoR1</span></td>
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      <td style="text-align: right; width: 87px;">4μL</td>
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    </tr>
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    <tr>
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      <td style="width: 108px;"><span
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style="font-weight: bold;">Pst1</span></td>
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      <td style="text-align: right; width: 87px;">4μL</td>
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    </tr>
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    <tr>
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      <td style="width: 108px;">FD Green buffer</td>
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      <td style="text-align: right; width: 87px;">8μL</td>
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    </tr>
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    <tr>
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      <td style="width: 108px;">Sample</td>
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      <td style="text-align: right; width: 87px;">20μL</td>
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    </tr>
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  </tbody>
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</table>
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<br>
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<span style="font-style: italic;">pSB1C3</span><br>
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<meta content="text/html; charset=ISO-8859-1"
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http-equiv="content-type">
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<title></title>
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<table style="text-align: left; width: 212px; height: 142px;"
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border="1" cellpadding="2" cellspacing="2">
 +
  <tbody>
 +
    <tr>
 +
      <td style="width: 108px;">H<small><small><small><small>2<big><big><big><big>O</big></big></big></big></small></small></small></small></td>
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      <td style="text-align: right; width: 87px;">24μL</td>
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    </tr>
 +
    <tr>
 +
      <td style="width: 108px;"><span
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style="font-weight: bold;">EcoR1</span></td>
 +
      <td style="text-align: right; width: 87px;">2μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="width: 108px;"><span
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style="font-weight: bold;">Pst1</span></td>
 +
      <td style="text-align: right; width: 87px;">2μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="width: 108px;">FD Green buffer</td>
 +
      <td style="text-align: right; width: 87px;">4μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="width: 108px;">Sample</td>
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      <td style="text-align: right; width: 87px;">10μL</td>
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    </tr>
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  </tbody>
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</table>
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<br>
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<span style="font-weight: bold;">FlhDCmut and pSB1AK3
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Restriction digest<br>
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</span><span style="font-style: italic;">FlhDCmut</span><span
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style="font-weight: bold;"><br>
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</span>
 +
<meta content="text/html; charset=ISO-8859-1"
 +
http-equiv="content-type">
 +
<title></title>
 +
<table style="text-align: left; width: 212px;" border="1"
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cellpadding="2" cellspacing="2">
 +
  <tbody>
 +
    <tr>
 +
      <td style="width: 108px;">H<small><small><small><small>2<big><big><big><big>O</big></big></big></big></small></small></small></small></td>
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      <td style="text-align: right; width: 87px;">48μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="width: 108px;"><span
 +
style="font-weight: bold;">EcoR1</span></td>
 +
      <td style="text-align: right; width: 87px;">4μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="width: 108px;"><span
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style="font-weight: bold;">Spe1</span></td>
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      <td style="text-align: right; width: 87px;">4μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="width: 108px;">FD Green buffer</td>
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      <td style="text-align: right; width: 87px;">8μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="width: 108px;">Sample</td>
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      <td style="text-align: right; width: 87px;">20μL</td>
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    </tr>
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  </tbody>
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</table>
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<br>
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<span style="font-style: italic;">pSB1AK3</span><br>
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<meta content="text/html; charset=ISO-8859-1"
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http-equiv="content-type">
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<title></title>
 +
<table style="text-align: left; width: 212px;" border="1"
 +
cellpadding="2" cellspacing="2">
 +
  <tbody>
 +
    <tr>
 +
      <td style="width: 108px;">H<small><small><small><small>2<big><big><big><big>O</big></big></big></big></small></small></small></small></td>
 +
      <td style="text-align: right; width: 87px;">24μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="width: 108px;"><span
 +
style="font-weight: bold;">EcoR1</span></td>
 +
      <td style="text-align: right; width: 87px;">2μL</td>
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    </tr>
 +
    <tr>
 +
      <td style="width: 108px;"><span
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style="font-weight: bold;">Xba1</span></td>
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      <td style="text-align: right; width: 87px;">2μL</td>
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    </tr>
 +
    <tr>
 +
      <td style="width: 108px;">FD Green buffer</td>
 +
      <td style="text-align: right; width: 87px;">4μL</td>
 +
    </tr>
 +
    <tr>
 +
      <td style="width: 108px;">Sample</td>
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      <td style="text-align: right; width: 87px;">10μL</td>
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    </tr>
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  </tbody>
 +
</table>
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<br>
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<br>
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</body>
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</html>
'''Results:''' <Br>
'''Results:''' <Br>
<Br><Br>
<Br><Br>
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=== Ligation of FlhDCmut and pSB1C3 ===
=== Ligation of FlhDCmut and pSB1C3 ===

Revision as of 17:50, 12 September 2010

Retrieved from "http://2010.igem.org/Team:SDU-Denmark/labnotes9"