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Team:Newcastle/10 September 2010
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Swoodhouse (Talk | contribs) (→Miniprep of BBa_I13401 (GFP coding sequence + terminator)) |
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==Double digests== | ==Double digests== | ||
| + | .. double digests for standard BioBrick assembly.. cut the arabinose-inducible promoter BioBrick with EcoRI and SpeI, cut gfp+terminator BioBrick with EcoRI and XbaI, for later ligation.. | ||
==Gel extraction of digests== | ==Gel extraction of digests== | ||
Revision as of 10:23, 10 September 2010
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Contents |
Arabinose-inducible promoter characterisation
...
Miniprep of BBa_I13401 (GFP coding sequence + terminator)
..from last night's overnight cultures from the 8th of september transformation.
Double digests
.. double digests for standard BioBrick assembly.. cut the arabinose-inducible promoter BioBrick with EcoRI and SpeI, cut gfp+terminator BioBrick with EcoRI and XbaI, for later ligation..
Gel extraction of digests
Ligation
   
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