"
Team:TU Delft/8 September 2010 content
From 2010.igem.org
(Difference between revisions)
(New page: Yay! Colonies on our Top10 plates! We're running a colony PCR as we speak to check if they contain the desired insert... stay tuned) |
|||
| Line 1: | Line 1: | ||
Yay! Colonies on our Top10 plates! We're running a colony PCR as we speak to check if they contain the desired insert... stay tuned | Yay! Colonies on our Top10 plates! We're running a colony PCR as we speak to check if they contain the desired insert... stay tuned | ||
| + | |||
| + | ==Emulsifier== | ||
| + | Our new construct with AlnA should be shipped by now by MrGene. Meanwhile we have optimized the protocol of the emulsification assay. In addition to the [[Team:TU_Delft#page=pages/blog&blog=31_August_2010|assay done last week]], I now repeated the assay using only M9 and LB. This was done to see whether the medium itself already shows some background in the assay. | ||
| + | |||
| + | The experiment was done in triplicate. | ||
| + | |||
| + | [[Image:TU_Delft_emulsification_assay_M9_LB.GIF]] | ||
| + | |||
| + | Since the background was already 0.223, opposed to about 0.12 last time, the emulsification capacity of M9 seems to be in the 0.5 mM SDS range. LB shows a higher emulsification capacity. So we decided to use K12 as the host for our emulsification production. | ||
Revision as of 11:47, 8 September 2010
Yay! Colonies on our Top10 plates! We're running a colony PCR as we speak to check if they contain the desired insert... stay tuned
Emulsifier
Our new construct with AlnA should be shipped by now by MrGene. Meanwhile we have optimized the protocol of the emulsification assay. In addition to the assay done last week, I now repeated the assay using only M9 and LB. This was done to see whether the medium itself already shows some background in the assay.
The experiment was done in triplicate.
Since the background was already 0.223, opposed to about 0.12 last time, the emulsification capacity of M9 seems to be in the 0.5 mM SDS range. LB shows a higher emulsification capacity. So we decided to use K12 as the host for our emulsification production.
