Team:UNIPV-Pavia/Calendar/September/settimana2

From 2010.igem.org

(Difference between revisions)
(→September, 7th)
(→September, 7th)
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|''' Culture'''||'''Quantifications (ng/ul)'''
|''' Culture'''||'''Quantifications (ng/ul)'''
|-
|-
-
| <partinfo>BBa_J23100</partinfo>_4C5 ||
+
| <partinfo>BBa_J23100</partinfo>_4C5 || 11,8
|-
|-
-
| <partinfo>BBa_J23101</partinfo>_4C5 ||
+
| <partinfo>BBa_J23101</partinfo>_4C5 || 39,7
|-
|-
-
| <partinfo>BBa_J23105</partinfo>_4C5 ||
+
| <partinfo>BBa_J23105</partinfo>_4C5 || 25,4
|-
|-
-
| <partinfo>BBa_J23106</partinfo>_4C5 ||
+
| <partinfo>BBa_J23106</partinfo>_4C5 || 29,7
|-
|-
-
| <partinfo>BBa_J23110</partinfo>_4C5 ||
+
| <partinfo>BBa_J23110</partinfo>_4C5 || 24,7
|-
|-
-
| <partinfo>BBa_J23114</partinfo>_4C5 ||
+
| <partinfo>BBa_J23114</partinfo>_4C5 || 15,4
|-
|-
-
| <partinfo>BBa_J23116</partinfo>_4C5 ||
+
| <partinfo>BBa_J23116</partinfo>_4C5 || 20,7
|-
|-
-
| <partinfo>BBa_J23118</partinfo>_4C5 ||
+
| <partinfo>BBa_J23118</partinfo>_4C5 || 20,4
|-
|-
-
| I7-A ||
+
| I7-A || 113,6
|-
|-
-
| I8-5-D ||
+
| I8-5-D || 127,6
|-
|-
-
| I44-2 (Cm 34) ||
+
| I44-2 (Cm 34) || 63,9
|-
|-
-
| I51-3 (Cm 34) ||
+
| I51-3 (Cm 34) || 100,4
|-
|-
-
| I44-2 (Cm 12,5) ||
+
| I44-2 (Cm 12,5) || 68,1
|-
|-
-
| I51-3 (Cm 12,5) ||
+
| I51-3 (Cm 12,5) || 135,4
 +
|-
 +
| I47 || 74,8
|}
|}

Revision as of 11:05, 7 September 2010
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Week 1

Week 2

Week 3

Week 4

Week 5


SEPTEMBER: WEEK 2


September, 6th

Inoculum of I47, I48, I49 into 5 ml LB+Amp for TECAN experiment of the following day.

  • Inoculum of I44-2 and I51-3 into 5 ml LB+Cm34 to evaluate MiniPrep yields.
  • Inoculum of I44-2 and I51-3 into 5 ml LB+Cm12,5 to evaluate MiniPrep yields and for sequencing.
  • Inoculum for MiniPrep and sequencing of:
    • <partinfo>BBa_J23100</partinfo>_4C5
    • <partinfo>BBa_J23101</partinfo>_4C5
    • <partinfo>BBa_J23105</partinfo>_4C5
    • <partinfo>BBa_J23106</partinfo>_4C5
    • <partinfo>BBa_J23110</partinfo>_4C5
    • <partinfo>BBa_J23114</partinfo>_4C5
    • <partinfo>BBa_J23116</partinfo>_4C5
    • <partinfo>BBa_J23118</partinfo>_4C5
    • I7-A
    • I8-5-D

Inoculum of cultures for TECAN test on self-inducible promoters:

  • I14-1
  • I15-1
  • I16-1
  • I17-1
  • I18-1
  • I19-1
  • I14_4C5-2
  • I15_4C5-2
  • I16_4C5-1
  • I17_4C5-1
  • I18_4C5-1
  • I19_4C5-1
  • I6-3
  • I3-1
  • <partinfo>BBa_B0031</partinfo>


September, 7th

MiniPrep for:

CultureQuantifications (ng/ul)
<partinfo>BBa_J23100</partinfo>_4C5 11,8
<partinfo>BBa_J23101</partinfo>_4C5 39,7
<partinfo>BBa_J23105</partinfo>_4C5 25,4
<partinfo>BBa_J23106</partinfo>_4C5 29,7
<partinfo>BBa_J23110</partinfo>_4C5 24,7
<partinfo>BBa_J23114</partinfo>_4C5 15,4
<partinfo>BBa_J23116</partinfo>_4C5 20,7
<partinfo>BBa_J23118</partinfo>_4C5 20,4
I7-A 113,6
I8-5-D 127,6
I44-2 (Cm 34) 63,9
I51-3 (Cm 34) 100,4
I44-2 (Cm 12,5) 68,1
I51-3 (Cm 12,5) 135,4
I47 74,8

Cultures for TECAN test were diluted in the morning (h10:20) 1:100 (40ul in 4ml) and let grown at 37°C 220 rpm till they reach the desired OD.

September, 8th

September, 9th

September, 10th

September, 11th



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