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Team:KAIST-Korea/Notebook/Diary/September
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| + | == September 1 == | ||
| + | pREP41 vector has arrived. | ||
| + | -> we will do E.coli transformation and increase its number. | ||
| + | pREP42-GFP vector has arrived (in E.coli) | ||
| + | -> will incubate a day and do spreading for further experiment. | ||
| + | |||
| + | Making culture media | ||
| + | # 500mL LB broth + agar 1.5% (7.5g) | ||
| + | # Do auto clave | ||
| + | # Cool it down until it reaches 50~60℃ | ||
| + | # Add ampicillin(1000x 100mg/mL) and stir | ||
| + | # putting out bubbles on culture media | ||
| + | # pouling on petri dish and wait for 1 hour | ||
| + | |||
| + | Culture | ||
| + | # Competent cell + DN10A and leave it in ice for 30min | ||
| + | # Do heat shock for 45 sec and store it in ice for 2min | ||
| + | # Stabilize it in incubator for 30 to 40min | ||
| + | # Do spreading | ||
Revision as of 07:27, 3 September 2010
September 1pREP41 vector has arrived. -> we will do E.coli transformation and increase its number. pREP42-GFP vector has arrived (in E.coli) -> will incubate a day and do spreading for further experiment. Making culture media
Culture
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