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Team:LMU-Munich/Notebook/Protocols/17 Special thermal cycler programs/Touch down
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Jara (Talk | contribs)
(New page: PCR program: Touch-Down ::{| |- |95°C |1 min |- |95°C |30 sec |- |56°C |60 sec |- |72°C |30 sec |- |return to step 2 for 2 cycles |- |95°C |30 sec |- |54°C |60 sec |- |72°C |30 sec...)
Newer edit →
(New page: PCR program: Touch-Down ::{| |- |95°C |1 min |- |95°C |30 sec |- |56°C |60 sec |- |72°C |30 sec |- |return to step 2 for 2 cycles |- |95°C |30 sec |- |54°C |60 sec |- |72°C |30 sec...)
Newer edit →
Revision as of 12:40, 1 September 2010
PCR program: Touch-Down
95°C 1 min 95°C 30 sec 56°C 60 sec 72°C 30 sec return to step 2 for 2 cycles 95°C 30 sec 54°C 60 sec 72°C 30 sec return to step 6 for 2 cycles 95°C 30 sec 52°C 60 sec 72°C 30 sec return to step 10 for 2 cycles 95°C 30 sec 50°C 60 sec 72°C 30 sec return to step 14 for 2 cycles 95°C 30 sec 48°C 60 sec 72°C 30 sec return to step 18 for 2 cycles 95°C 30 sec 46°C 60 sec 72°C 30 sec return to step 22 for 2 cycles 95°C 30 sec 44°C 60 sec 72°C 30 sec return to step 26 for 2 cycles 95°C 30 sec 42°C 60 sec 72°C 30 sec return to step 30 for 2 cycles 95°C 30 sec 48°C 60 sec 72°C 30 sec return to step 34 for 30 cycles 72°C 10 min 12°C forever
