Team:UNIPV-Pavia/Calendar/September/settimana1

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Revision as of 09:52, 31 August 2010

SEPTEMBER: WEEK 1

August, 30th

Colony PCR was performed on <partinfo>BBa_J23116</partinfo>_4C5 (3 colonies) and <partinfo>BBa_J23118</partinfo>_4C5 (3 colonies), since they were negative at the past PCR screening (see August, 27th). Gel results are shown in the image below. As you can see, all the clones were correct, so we kept the first colony for both and prepared glycerol stocks. <partinfo>BBa_J23116</partinfo>_4C5 and <partinfo>BBa_J23118</partinfo>_4C5 are stored at -80°C and soon will be tested with the other clones we prepared and screened last week.

PCR results for <partinfo>BBa_J23116</partinfo>_4C5 1, 2 and 3 and for <partinfo>BBa_J23118</partinfo>_4C5 1, 2, 3. The last lane is reaction blank.

Transformation of ligations I47, I48, I49 into E. coli DH5-alpha. Strains were plated on LB+Amp agar plates and let grow at 37°C.

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August, 31st

PCR from colony for I47, I48, I49 ligations. Three colonies were picked from each plate, and they were also let grow in LB+Amp to make glycerol stocks of the positive ones.

Colony PCR for I47, I48, I49 ligations.

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September, 1st

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September, 2nd

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September, 3rd

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September, 4th

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 ==August, 31st==
 PCR from colony for I47, I48, I49 ligations. Three colonies were picked from each plate, and they were also let grow in LB+Amp to make glycerol stocks of the positive ones.
+[[Image:UNIPV10_31_8_10_ColonyPCR_I47_I48_I49.jpg|thumb|200px|center|Colony PCR for I47, I48, I49 ligations.]]
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