"

From 2010.igem.org

Revision as of 04:53, 31 August 2010 (view source) Dannii (Talk | contribs) (→Taq DNA Polymerase) ← Older edit		Revision as of 05:11, 31 August 2010 (view source) Dannii (Talk | contribs) (→Taq DNA Polymerase) Newer edit →
Line 453:		Line 453:
	----		----
		+	Our Taq Polymerase with mutations
		+
		+
		+	485 Asparagine - Valine
		+
		+	515 Serine - Leucine
		+
		+	540 Lysine - Leucine
		+
		+	659 Arginine - Isolucine
		+
		+	663 Lysine - Leucine
		+
		+
		+
		+
		+	'''Aliphatic Index:''' 98.2
		+
		+	'''Ext Coefficient:''' 107260
		+
		+	'''Theoretical PI:''' 5.91
		+
		+	'''Instability Index:''' 32.91
	== Fold-it==		== Fold-it==

---

Revision as of 05:11, 31 August 2010

Modelling

Taq DNA Polymerase

Taq Polymerase with no mutations

Aliphatic Index: 98.33

Ext Coefficient: 112760

Theoretical PI: 6.04

Instability Index: 32.93

---

We redesigned TAQ so it is suitable to undergo ligation independent cloning, to do this we changed the N and C terminal.

This is the redesigned Taq Polymerase

Aliphatic Index: 96.46

Ext Coefficient: 107260

Theoretical PI: 6.09

Instability Index: 33.39

The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins.

The picture below shows the differences between the wild type Taq Polymerase and the redesigned Taq.

(Green is the redesigned Taq and Pink is the wild type Taq)

---

Our Taq Polymerase with mutations

485 Asparagine - Valine

515 Serine - Leucine

540 Lysine - Leucine

659 Arginine - Isolucine

663 Lysine - Leucine

Aliphatic Index: 98.2

Ext Coefficient: 107260

Theoretical PI: 5.91

Instability Index: 32.91

Fold-it

Foldit is a revolutionary new computer game enabling you to contribute to important scientific research. This page describes the science behind Foldit and how your playing can help. [http://fold.it/portal/ Click Here]