Raida Today, I set up a PCR Reaction in order to biobrick MalE and MalE31 without the signalling sequence. I set up 8x with the MalE-BBKdelss-F and the MalE-R primers. The negative control is a MalE plasmid. Another set of reaction was set, which is with the MalE-BBk-F and the MalE-R and the positive control was MalE plasmid.

I have also completed my draft with suggestion made from Himika and Paul on my introduction and also which section should have the paragraphs that I had written. I also wrote for the Legal portion of the paper.

Jeremy

Today is my first real day of wetlab stuff since my time off. I redid the pRFP biobrick and will run the gel tomorrow. I also did a transformation of MalE, MalE31 and NlpE into the Ravio competent cells. I also made more agar and broth.

None wetlab stuff that I did included fixing areas of the wiki, writing up the transcription/translation portion of the circuit and doing the Calgary, University of Calgary and O'brien sections of the wiki.