"
Team:UNIPV-Pavia/Calendar/September/settimana1
From 2010.igem.org
m (→August, 30th) |
(→August, 30th) |
||
| Line 37: | Line 37: | ||
Colony PCR was performed on <partinfo>BBa_J23116</partinfo>_4C5 (3 colonies) and <partinfo>BBa_J23118</partinfo>_4C5 (3 colonies), since they were negative at the past PCR screening (see August, 27th). | Colony PCR was performed on <partinfo>BBa_J23116</partinfo>_4C5 (3 colonies) and <partinfo>BBa_J23118</partinfo>_4C5 (3 colonies), since they were negative at the past PCR screening (see August, 27th). | ||
| + | Gel results are shown in the image below. As you can see, all the clones were correct, so we kept the first colony for both and prepared glycerol stocks. | ||
| + | <partinfo>BBa_J23116</partinfo>_4C5 and <partinfo>BBa_J23118</partinfo>_4C5 are stored at -80°C and soon will be tested with the other clones we prepared and screened last week. | ||
| + | |||
| + | [[Image:UNIPV10_PCR_J116_123_J118_123_blank.jpg|thumb|200px|center|PCR results for <partinfo>BBa_J23116</partinfo>_4C5 1, 2 and 3 and for <partinfo>BBa_J23118</partinfo>_4C5 1, 2, 3. The last lane is reaction blank.]] | ||
| + | |||
| + | |||
---- | ---- | ||
Revision as of 12:42, 30 August 2010
|
|
||||||||||||||||||||||||||||||
|
|
|||||||||||||||||||||||||||||
