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Team:Newcastle/27 August 2010
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===Materials and Protocol=== | ===Materials and Protocol=== | ||
| - | Please refer to the [[Team:Newcastle/Restriction_digests|restriction digest]] and [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]] protocols. | + | Please refer to the [[Team:Newcastle/Restriction_digests|restriction digest]], [[Team:Newcastle/PCR#Phusion_PCR|Phusion PCR]] and [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]] protocols. |
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Revision as of 10:36, 27 August 2010
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Contents |
PCR and digestion controls
Aims
Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that there is nothing wrong with any of our enzymes or ...
Materials and Protocol
Please refer to the restriction digest, Phusion PCR and gel electrophoresis protocols.
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Results
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Discussion
Worked correctly.. nothing is wrong with our enzymes.. in the case of our rocF plasmid PCRs, it must be the primers which are the problem.. new ones are supposed to be arriving today.. in the other cases, repeat..
yneA
   
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