pSB1C3 plasmid gel electrophoresis

Aims

The aim of this experiment is to run gel electrophoresis for the extracted and linearized plasmid pSB1C3 fragment which were amplified at 4 different melting temperatures by 4 separate PCR reactions.

Materials and Protocol

Please refer to: Gel electrophoresis for gel electrophoresis protocol.

Result

Figure 1: Gel electrophoresis of plasmid pSB1C3 linearized fragments which are amplified by 4 different PCR reactions running at 4 different melting temperatures. A 1 kb DNA ladder

• Lane 1: pSB1C3 fragment amplified at 55°C
• Lane 2: pSB1C3 fragment amplified at 55°C
• Lane 3: pSB1C3 fragment amplified at 60°C
• Lane 4: pSB1C3 fragment amplified at 60°C
• Lane 5: pSB1C3 fragment amplified at 65°C
• Lane 6: pSB1C3 fragment amplified at 65°C
• Lane 7: pSB1C3 fragment amplified at 70°C
• Lane 8: pSB1C3 fragment amplified at 70°C

Discussion

Conclusion