1st solid idea: respiratory system using spike protein & HNMT
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1st solid idea: respiratory system using spike protein & HNMT<br>
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We have to make two plasmid which would be guide plasmid and functional plasmid. We put spike gene in guide plasmid and HNMT in functional plasmid. Then bacteria will get spikes on outer membrane and HNMT inside the cell. After that if we can pack HNMT in the vesicle. Then we can make a DDS. And we can check if HNMT works by using quantum dot or GFP.
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We have to make two plasmid, which would be a guide plasmid and a functional plasmid. We put spike gene in the guide plasmid and HNMT in the functional plasmid. Then, bacteria will get spikes on the outer membrane and HNMT inside the cell. After that, if we can pack HNMT in the vesicle, we can make a DDS. And we can check if HNMT works by using quantum dot or GFP.
: Do list
: Do list
Revision as of 04:09, 16 August 2010
May 26
Make spike and HNMT plasmid and transform bacteria with them. Bacteria expressing them will produce HNMT and engulf via membrane fusion. Ultimately, use the bacteria in a spray.
What we should do (June 1st~August 31th)
Syenthesis of bacteria
Company: S.B. Yang>contact, rest>look for companies. Contact T.A.
Sequence source: D.C. Yang, K.K. Kim
1),2): by June 1st
KI building (Prof. Choi)
Homepage design
K.K. Kim, +alpha
SPARCS
I.D. department
2),3): cost a lot - about \1million. stick with 1)
S.B. Yang, K.K. Kim, D.C. Yang, H.S. Kim handle program until June 6th. The rest (design team) design.
Daily update
Confirmation of the product
Florescent material such as GFP
Quantum dot
May 27
1st solid idea: respiratory system using spike protein & HNMT
We have to make two plasmid, which would be a guide plasmid and a functional plasmid. We put spike gene in the guide plasmid and HNMT in the functional plasmid. Then, bacteria will get spikes on the outer membrane and HNMT inside the cell. After that, if we can pack HNMT in the vesicle, we can make a DDS. And we can check if HNMT works by using quantum dot or GFP.
Do list
See Stanford-secretion gene (everyone)
Find potential genes
Literature: H.S. Kim, S.H. Kim, S.J. Kim, Triple Yangs
Company: rest
by Saturday
KI workshop next Friday
DNA kit will arrive. Homepage design team assigned.
Potential name: FADEX (Fast Accurate Drug Exporting System)
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