Apoptosis Notebook

Contents

8-02-2010

• Some test text here.

8-03-2010

Some test text in bold We created following tests:

• test1
• test2
• test3

8-04-2010

Example of a table

8-05-2010

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8-06-2010

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8-07-2010

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8-08-2010

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8-09-2010

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8-10-2010

Transforming competent cells

- eGFP Biobrick: BBa_I714891 SDY_eGFP (Kanamycin)

- TEV recogn N Degron SF3 = pDS7 (Ampicillin)

- TEV p14 recogn = 190-6 (Ampicillin)

-> Protocol: https://www.openwetware.org/wiki/Transforming_chemically_competent_cells_protocol (3 Transformation)

- We added 2 µl DNA

- We plated out 200 µl

Plasmid Isolation

- CMV-Promoter Biobrick: BBa_J52034

-> Protocol: http://www.promega.com/protcards/9fb093/fb093.pdf (4 Plasmid extraction from cells)

- Prepared overnight culture, measured concentration of DNA

-> Poor results -> thrown away

8-11-2010

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8-12-2010

- plasmid extraction of pDS7 (458ng/µl), eGFP (55ng/µl), 190-6 (193ng/µl)

-> Protocol: (4 Plasmid extraktion from cells)

- Restriction digest with EcoRI and PstI in buffer H (for testing DNA is correct)

       10µg DNA: pDS7 (2µl), eGFP (15µl), 190-6 (10µl)

-> Protocol: (5 Restriction digest)

- Colony for Plasmidextraktion (CMV, eGFP, pDS7, 190-6) plated

- PhiC31o plated on Ampicillin-Agar, stored at 37°C

- 50% Glycerol made (for the glycerolstock PhiC31o)

8-13-2010

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8-14-2010

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8-15-2010

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8-16-2010

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8-17-2010

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8-18-2010

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8-19-2010

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8-20-2010

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8-21-2010

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8-22-2010

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