Team:Edinburgh/Bacterial/Green light producer

From 2010.igem.org

(Difference between revisions)

Revision as of 11:27, 12 August 2010

Overview: The green light producer

The green luciferase will require the combination of luxAB with the production of another protein: YFP from ''Vibrio fischeri''. This will shift the wavelength of the light towards the yellow spectrum, making it the correct colour to activate our green light sensor. Mexico UNAM-Genomics have had this DNA sequence synthesised and sending it to us for characterisation and combination with luxAB. We also expect Mexico to BioBrick it as part of their submission to the registry.

Problems

You can't have problems with a piece of DNA you've never met.

Biobricks

References

Fujii, H., K. Noda, et al. (2007). "Increase in bioluminescence intensity of firefly luciferase using genetic modification." Analytical Biochemistry 366(2): 131-136.

@@ Line 119: / Line 119: @@
 <br>
-<p>The green luciferase will require the combination of luxAB with the production of another protein: YFP from ''Vibrio fischeri''. This will shift the wavelength of the light towards the yellow spectrum, making it the correct colour to activate our green light sensor. We are having this DNA sequence synthesised and hope to BioBrick it as part of our submission to the registry.</p>
+<p>The green luciferase will require the combination of luxAB with the production of another protein: YFP from ''Vibrio fischeri''. This will shift the wavelength of the light towards the yellow spectrum, making it the correct colour to activate our green light sensor. Mexico UNAM-Genomics have had this DNA sequence synthesised and sending it to us for characterisation and combination with luxAB. We also expect Mexico to BioBrick it as part of their submission to the registry.</p>
-<p>Since the wild type luciferase naturally emits in the green light part of the visible spectrum, it could also potentially excite a green light sensor. Since the limiting factor for light communication between bacteria is probably the amount of light produced, we are planning on introducing 3 mutations into the luciferase that increase the brightness in vitro by a factor of 12.5. We are getting this part synthesised, which will also optimise codons for expression in E coli, which should help to increase the amount of light emitted. (see Fujii et al, 2007). </p>
 <br>
@@ Line 128: / Line 127: @@
 <a name="Problems" id="Problems"></a><h2>Problems</h2>
 <br>
+You can't have problems with a piece of DNA you've never met.
 <p></p>