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Team:Newcastle/9 August 2010
From 2010.igem.org
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==Discussion== | ==Discussion== | ||
| - | * For pVeg, we will set up an [[Team:Newcastle/Growing_an_overnight_cultures|overnight broth culture]] from the colonies | + | * For pVeg, we will set up an [[Team:Newcastle/Growing_an_overnight_cultures|overnight broth culture]] from the colonies for [[Team:Newcastle/Qiagen_Minipreps|plasmid extraction]] |
* To show that we have sucessfully transformed from ''lacI'', we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures. | * To show that we have sucessfully transformed from ''lacI'', we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures. | ||
Revision as of 10:55, 9 August 2010
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Contents |
Transformation of pVeg and lacI
Results/Aims
- There are colonies on all 3 plates for pVeg.
- For lacI, there is only one colony on one of the normal plates and more on the concentrated one.
Discussion
- For pVeg, we will set up an overnight broth culture from the colonies for plasmid extraction
- To show that we have sucessfully transformed from lacI, we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
Transformation of lacI
Aims
To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.
Materials and Protocol
Please refer to Transformation of E.coli.
Result
Please refer to 10.08.10 for result.
   
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