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Team:Newcastle/9 August 2010
From 2010.igem.org
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| - | * pVeg was successfully transformed- From this we will set up an overnight broth culture for plasmid extraction | + | * pVeg was successfully transformed - From this we will set up an overnight broth culture for plasmid extraction |
* ''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures. | * ''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures. | ||
Revision as of 10:27, 9 August 2010
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Contents |
Transformation
Results/Aims
- pVeg was successfully transformed - From this we will set up an overnight broth culture for plasmid extraction
- lacI- To show that we have sucessfully transformed from lacI we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
Transformation of lacI
Aims
To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.
Materials and Protocol
Please refer to Transformation of E.coli.
Result
Please refer to 10.08.10 for result.
   
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