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Team:Newcastle/9 August 2010
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*''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture. | *''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture. | ||
| - | =Transformation | + | =Transformation of lacI= |
==Aims== | ==Aims== | ||
To transform some competent ''E.coli'' DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on [[Team:Newcastle/6_August_2010|06.08.10]]. | To transform some competent ''E.coli'' DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on [[Team:Newcastle/6_August_2010|06.08.10]]. | ||
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| + | ==Materials and Protocol== | ||
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| + | Please refer to [[TeamNewcastleTransformation_of_E._coli0|Transformation of E.coli]]. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 10:18, 9 August 2010
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Contents |
Transformation
Results/Aims
- pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
- lacI- To show that we have sucessfully transformed from lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
Transformation of lacI
Aims
To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.
Materials and Protocol
Please refer to Transformation of E.coli.
   
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